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验证数据展示

  • WB analysis using 87677-3-RR (same clone as 87677-3-PBS)

    WB analysis using 87677-3-RR (same clone as 87677-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 87677-3-RR (SIRT6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 87677-3-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 87677-3-RR (same clone as 87677-3-PBS)

    IP experiment of HeLa using 87677-3-RR (same clone as 87677-3-PBS)

    IP result of anti-SIRT6 (IP:87677-3-RR, 4ug; Detection:87677-3-RR 1:2000) with HeLa cells lysate 920 ug. This data was developed using the same antibody clone with 87677-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 87677-3-RR (same clone as 87677-3-PBS)

    IHC staining of human colon using 87677-3-RR (same clone as 87677-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 87677-3-RR (SIRT6 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 87677-3-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIRT6 antibody included

SIRT6 Recombinant monoclonal antibody, PBS Only

SIRT6 Uni-rAb® Recombinant Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 87677-3-PBS

产品说明书

CloneNo. 253025G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, hSIRT6, NAD-dependent protein deacetylase sirtuin-6

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SIRT6 Ready-To-Use IHC Kit. SIRT6 primary antibody included.

产品信息

87677-3-PBS targets SIRT6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4469

Product name: Recombinant human SIRT6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-328 aa of BC028220

Sequence: MSVNYAAGLSPYADKGKCGLPEIFDPPEELERKVWELARLVWQSSNVVFHTGAGISTASGIPDFRGPHGVWTMEERGLAPKFDTTFESARPTQTHMALVQLERVGLLRFLVSQNVDGLHVRSGFPRDKLAELHGNMFVEECAKCKTQYVRDTVVGTMGLKATGRLCTVAKARGLRACRNADLSITLGTSLQIRPSGNLPLATKRRGGRLVIVNLQPTKHDRHADLRIHGYVDEVMTRLMKHLGLEIPAWDGPRVLERALPPLPRPPTPKLEPKEESPTRINGSIPAGPKQEPCAQHNGSEPASPKRERPTSPAPHRPPKRVKAKAVPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
别名 EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, hSIRT6, NAD-dependent protein deacetylase sirtuin-6
计算分子量 39 kDa
观测分子量 37-45 kDa
GenBank蛋白编号BC028220
基因名称 SIRT6
Gene ID (NCBI) 51548
RRIDAB_3745691
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ8N6T7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT6, also named as SIR2L6, belongs to the sirtuin family. It is known to deacetylate H3K9, H3K56 and CtIP in vivo. SIRT6 regulates the expression of a large number of stress-responsive and metabolism related genes, promotes genomic stability and stimulates base excision and double strand break DNA repair(PMID: 20729089). By maintaining both the integrity and the expression of the mammalian genome, SIRT6 thus serves several roles that parallel Sir2 function(PMID: 20729089). In addtion, Sirt6-deficient animals develop normally for several weeks after birth, but succumb to an acute multi-organ degenerative syndrome that is uniformly lethal by one month of age(PMID: 19135889).

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