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验证数据展示

  • WB analysis using 28677-1-AP

    WB analysis using 28677-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28677-1-AP (SIRT6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 28677-1-AP

    WB analysis of HeLa using 28677-1-AP

    WB result of SIRT6 antibody (28677-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIRT6 transfected HeLa cells.

  • IHC staining of mouse liver using 28677-1-AP

    IHC staining of mouse liver using 28677-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28677-1-AP (SIRT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SIRT6 antibody included
  • IHC staining of human placenta using 28677-1-AP

    IHC staining of human placenta using 28677-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28677-1-AP (SIRT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SIRT6 antibody included
  • IHC staining of rat liver using 28677-1-AP

    IHC staining of rat liver using 28677-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 28677-1-AP (SIRT6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SIRT6 antibody included
  • IF Staining of SH-SY5Y using 28677-1-AP

    IF Staining of SH-SY5Y using 28677-1-AP

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using SIRT6 antibody (28677-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Featured Product
  • KD/KO验证

SIRT6 Polyclonal antibody

SIRT6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28677-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, hSIRT6, NAD-dependent protein deacetylase sirtuin-6

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, PC-3 cells
Positive IHC detected inhuman placenta tissue, mouse colon tissue, mouse liver tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy SIRT6 Ready-To-Use IHC Kit. SIRT6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28677-1-AP targets SIRT6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SIRT6 fusion protein Ag29163 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 6 (S. cerevisiae)
别名 EC:2.3.1.-, EC:2.3.1.286, EC:2.4.2.-, hSIRT6, NAD-dependent protein deacetylase sirtuin-6
计算分子量 39 kDa
观测分子量39 kDa
GenBank蛋白编号BC028220
基因名称 SIRT6
Gene ID (NCBI) 51548
RRIDAB_3669666
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N6T7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SIRT6 gene is located at chromosome 19p13.3 and contains 8 exons. The encoded protein has a total length of 355 amino acids. SIRT6 protein is a member of the Sirtuins, which is a class of NAD+-dependent protein deacetylases involved in stress resistance and metabolic homeostasis. SIRT6 also plays roles in various tumors as both an oncogene and tumor suppressor gene. A previous study in osteosarcoma reported that SIRT6 regulates the migration and invasion of tumors through the ERK1/2/MMP9 pathway. (PMID: 30675128)

实验方案

Product Specific Protocols
WB protocol for SIRT6 antibody 28677-1-APDownload protocol
IHC protocol for SIRT6 antibody 28677-1-APDownload protocol
IF protocol for SIRT6 antibody 28677-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols