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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 82246-1-RR (same clone as 82246-1-PBS)

    WB analysis using 82246-1-RR (same clone as 82246-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82246-1-RR (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82246-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82246-1-RR (same clone as 82246-1-PBS)

    IF Staining of HeLa using 82246-1-RR (same clone as 82246-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIRT5 antibody (82246-1-RR, Clone: 7L20 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82246-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82246-1-RR

    Affinity and Kinetic Characterization of 82246-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82246-1-RR against Human SIRT5 were performed. The affinity constant is 1.64 nM.

SIRT5 Recombinant antibody, PBS Only

SIRT5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 82246-1-PBS

产品说明书

CloneNo. 7L20

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

7L20, EC:2.3.1.-, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2L5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82246-1-PBS targets SIRT5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 SIRT5 fusion protein Ag7195 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
别名 7L20, EC:2.3.1.-, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2L5
计算分子量 34 kDa
观测分子量 32-33 kDa
GenBank蛋白编号BC000126
基因名称 SIRT5
Gene ID (NCBI) 23408
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NXA8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and this contributes to the regulation of blood ammonia levels.