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SIRPD Polyclonal antibody

SIRPD Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 26594-1-AP

Print datasheet

Synonyms

PTPNS1L2, SIRP delta, SIRPD

验证数据展示

  • WB analysis of fetal human brain using 26594-1-AP

    WB analysis of fetal human brain using 26594-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 26594-1-AP (SIRPD antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 26594-1-AP

    IHC staining of human brain using 26594-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26594-1-AP (SIRPD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 26594-1-AP

    IHC staining of human brain using 26594-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26594-1-AP (SIRPD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected infetal human brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26594-1-AP is SIRPD Fusion Protein expressed in E. coli.

Tested Applications IHC, WB,ELISA
Tested Reactivity human
Immunogen SIRPD fusion protein Ag24246 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal-regulatory protein delta
Synonyms PTPNS1L2, SIRP delta, SIRPD
Calculated Molecular Weight 197 aa, 22 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC033502
Gene Symbol SIRPD
Gene ID (NCBI) 128646
RRIDAB_2880567
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIRPD antibody 26594-1-APDownload protocol
IHC protocol for SIRPD antibody 26594-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols