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验证数据展示

  • IF Staining of THP-1 using 83729-3-RR (same clone as 83729-3-PBS)

    IF Staining of THP-1 using 83729-3-RR (same clone as 83729-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using SIRP Alpha/CD172a antibody (83729-3-RR, Clone: 240789E7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83729-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83729-3-RR

    Affinity and Kinetic Characterization of 83729-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83729-3-RR against Human SIRP Alpha were performed. The affinity constant is 1.37 nM.

SIRP Alpha/CD172a Recombinant antibody, PBS Only

SIRP Alpha/CD172a Uni-rAbTM Recombinant Antibody for IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 83729-3-PBS

产品说明书

CloneNo. 240789E7

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, Cytometric bead array, Indirect ELISA

别名

SIRP Alpha, SIRPA, 240789E7, BIT, Brain Ig-like molecule with tyrosine-based activation motifs

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83729-3-PBS targets SIRP Alpha/CD172a in IF/ICC, Cytometric bead array, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 SIRP Alpha/CD172a fusion protein Eg0625 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal-regulatory protein alpha
别名 SIRP Alpha, SIRPA, 240789E7, BIT, Brain Ig-like molecule with tyrosine-based activation motifs
计算分子量55 kDa
GenBank蛋白编号NM_080792
基因名称 SIRP Alpha
Gene ID (NCBI) 140885
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP78324
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).