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验证数据展示

  • WB analysis using 68206-1-Ig (same clone as 68206-1-PBS)

    WB analysis using 68206-1-Ig (same clone as 68206-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68206-1-Ig (SIRP Beta 1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68206-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 68206-1-Ig (same clone as 68206-1-PBS)

    IHC staining of human lung cancer using 68206-1-Ig (same clone as 68206-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 68206-1-Ig (SIRP Beta 1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68206-1-PBS in a different storage buffer formulation.

  • IF Staining of OS-RC-2 using 68206-1-Ig (same clone as 68206-1-PBS)

    IF Staining of OS-RC-2 using 68206-1-Ig (same clone as 68206-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed OS-RC-2 cells using SIRP Beta 1 antibody (68206-1-Ig, Clone: 3A3H1 ) at dilution of 1:1000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 68206-1-PBS in a different storage buffer formulation.

SIRP beta 1/CD172b Monoclonal antibody, PBS Only

SIRP beta 1/CD172b Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 68206-1-PBS

产品说明书

CloneNo. 3A3H1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

SIRPB1, SIRP Beta 1, SIRP-beta-1, Signal-regulatory protein beta-1, CD172b

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

68206-1-PBS targets SIRP beta 1/CD172b in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag28615

Product name: Recombinant human SIRPB1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-181 aa of BC025286

Sequence: MPVPASWPHLPSPFLLMTLLLGRLTGVAGEDELQVIQPEKSVSVAAGESATLRCAMTSLIPVGPIMWFRGAGAGRELIYNQKEGHFPRVTTVSELTKRNNLDFSISISNITPADAGTYYCVKFRKGSPDDVEFKSGAGTELSVREPALAPTAPLLVALLLGPKLLLVVGVSAIYICWKQKA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 signal-regulatory protein beta 1
别名 SIRPB1, SIRP Beta 1, SIRP-beta-1, Signal-regulatory protein beta-1, CD172b
计算分子量 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC025286
基因名称 SIRPB1
Gene ID (NCBI) 10326
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO00241
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.

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