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Conjugate
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验证数据展示

  • WB analysis using 67337-1-Ig (same clone as 67337-1-PBS)

    WB analysis using 67337-1-Ig (same clone as 67337-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67337-1-Ig (SIRP Alpha/CD172a antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 67337-1-Ig (same clone as 67337-1-PBS)

    IHC staining of rat cerebellum using 67337-1-Ig (same clone as 67337-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 67337-1-Ig (SIRP Alpha antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67337-1-PBS in a different storage buffer formulation.

SIRP Alpha/CD172a Monoclonal antibody, PBS Only

SIRP Alpha/CD172a Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67337-1-PBS

产品说明书

CloneNo. 2F8H3

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, Indirect ELISA

别名

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67337-1-PBS targets SIRP Alpha/CD172a in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag6361

Product name: Recombinant human SIRPA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 158-508 aa of BC033092

Sequence: ARATPQHTVSFTCESHGFSPRDITLKWFKNGNELSDFQTNVDPVGESVSYSIHSTAKVVLTREDVHSQVICEVAHVTLQGDPLRGTANLSETIRVPPTLEVTQQPVRAENQVNVTCQVRKFYPQRLQLTWLENGNVSRTETASTVTENKDGTYNWMSWLLVNVSAHRDDVKLTCQVEHDGQPAVSKSHDLKVSAHPKEQGSNTAAENTGSNERNIYIVVGVVCTLLVALLMAALYLVRIRQKKAQGSTSSTRLHEPEKNAREITQVQSLDTNDITYADLNLPKGKKPAPQAAEPNNHTEYASIQTSPQPASEDTLTYADLDMVHLNRTPKQPAPKPEPSFSEYASVQVPRK

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 signal-regulatory protein alpha
别名 SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A
计算分子量 55 kDa
观测分子量 75-85 kDa
GenBank蛋白编号BC033092
基因名称 SIRP Alpha
Gene ID (NCBI) 140885
RRIDAB_2882595
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP78324
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954). This antibody detects a 75-85 kDa band.

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