验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, HEK-293T cells |
| Positive IHC detected in | mouse lung tissue, human ovary cancer tissue, rat lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26793-1-AP targets SIPA1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag25383 Product name: Recombinant human SIPA1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 950-1042 aa of BC010492 Sequence: GQPIPESGDPKGTPKSDAEPEPGNLSEKVSHLESMLRKLQEDLQKEKADRAALEEEVRSLRHNNRRLQAESESAATRLLLASKQLGSPTADLA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | signal-induced proliferation-associated 1 |
| 别名 | GTPase-activating protein Spa-1, p130 SPA 1, p130 SPA-1, Signal-induced proliferation-associated protein 1, Sipa 1 |
| 计算分子量 | 1042 aa, 112 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC010492 |
| 基因名称 | SIPA1 |
| Gene ID (NCBI) | 6494 |
| RRID | AB_2880637 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96FS4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIPA1 (Signal-induced proliferation-associated protein 1) is also named as SPA1, p130 SPA-1 and GTPase-activating protein Spa-1. SIPA1, a GTPase activating protein, was discovered in proliferating lymphocytes as mitogen-induced nuclear protein. SIPA1 promotes catalyzation of hydrolyze Rap1GTP/GDP and is known to be a negative regulator of Ras-related protein, which transduces the signals for various cellular functions, including development, cellular proliferation, and cellular adhesion (PMID:28237246). Transcription of fibronectin 1, which is crucial for cell junction and migration of triple-negative breast cancer (TNBC) cells, was regulated by SIPA1 in a DBR-dependent manner. SIPA1 was highly expressed in metastatic TNBC. SIPA1 served as a TF, promoting TNBC migration, invasion, and metastasis (PMID: 37500797).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SIPA1 antibody 26793-1-AP | Download protocol |
| WB protocol for SIPA1 antibody 26793-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Lett A TRIM21-UCHL3-ITCH-SIPA1 axis promotes colorectal cancer growth and metastasis.
| ||
Mol Cell Proteomics Characterization of Usher Syndrome Type 2-Associated Proteins in the Retina via Affinity Purification-Mass Spectrometry. | ||
Transl Oncol Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics. |
