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SIPA1 Polyclonal antibody

SIPA1 Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 26793-1-AP

Print datasheet

Synonyms

p130 SPA 1, Sipa 1, SIPA1, SPA1

验证数据展示

  • WB analysis of Raji using 26793-1-AP

    WB analysis of Raji using 26793-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 26793-1-AP (SIPA1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 26793-1-AP

    IF Staining of A431 using 26793-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using 26793-1-AP (SIPA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inRaji cells, HEK-293T cells
Positive IF detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26793-1-AP is SIPA1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen SIPA1 fusion protein Ag25383 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name signal-induced proliferation-associated 1
Synonyms p130 SPA 1, Sipa 1, SIPA1, SPA1
Calculated Molecular Weight 1042 aa, 112 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC010492
Gene Symbol SIPA1
Gene ID (NCBI) 6494
RRIDAB_2880637
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96FS4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIPA1 antibody 26793-1-APDownload protocol
IF protocol for SIPA1 antibody 26793-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Transl Oncol

Identification of metastasis-associated exoDEPs in colorectal cancer using label-free proteomics.

Authors - Xinlu Liu
View Article