验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, rat brain tissue |
| Positive IP detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SIP1 Ready-To-Use IHC Kit. SIP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
21672-1-AP targets SIP1 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16500 Product name: Recombinant human SIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-280 aa of BC104968 Sequence: MRRAELAGLKTMAWVPAESAVEELMPRLLPVEPCDLTEGFDPSVPPRTPQEYLRRVQIEAAQCPDVVVAQIDPKKLKRKQSVNISLSGCQPAPEGYSPTLQWQQQQVAQFSTVRQNVNKHRSHWKSQQLDSNVTMPKSEDEEGWKKFCLGEKLCADGAVGPATNESPGIDYVQIGFPPLLSIVSRMNQATVTSVLEYLSNWFGERDFTPELGRWLYALLACLEKPLLPEAHSLIRQLARRCSEVRLLVDSKDDERVPALNLLICLVSRYFDQRDLADEPS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | survival of motor neuron protein interacting protein 1 |
| 别名 | Component of gems 2, Gemin 2, GEMIN2, SIP1, SIP1 delta, SMN interacting protein 1 |
| 计算分子量 | 280 aa, 32 kDa |
| 观测分子量 | 32-35 kDa |
| GenBank蛋白编号 | BC104968 |
| 基因名称 | SIP1 |
| Gene ID (NCBI) | 8487 |
| RRID | AB_10859249 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O14893 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for SIP1 antibody 21672-1-AP | Download protocol |
| WB protocol for SIP1 antibody 21672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Functional interaction between FUS and SMN underlies SMA-like splicing changes in wild-type hFUS mice. | ||
Neurobiol Dis UsnRNP trafficking is regulated by stress granules and compromised by mutant ALS proteins. | ||
J Mol Med (Berl) Hyper-SUMOylation of SMN induced by SENP2 deficiency decreases its stability and leads to spinal muscular atrophy-like pathology. | ||
Biomolecules (20S)G-Rh2 Inhibits NF-κB Regulated Epithelial-Mesenchymal Transition by Targeting Annexin A2. |
