验证数据展示
经过测试的应用
| Positive WB detected in | U-251 cells, MCF-7 cells, HaCaT cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85702-4-RR targets SIN1/MAPKAP1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | SIN1/MAPKAP1 fusion protein Ag7753 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | mitogen-activated protein kinase associated protein 1 |
| 别名 | MAPKAP1, MIP1, Mitogen-activated protein kinase 2-associated protein 1, SAPK interacting protein 1, SIN1 |
| 计算分子量 | 59 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC002326 |
| 基因名称 | MAPKAP1 |
| Gene ID (NCBI) | 79109 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BPZ7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mitogen-activated protein kinase 2-associated protein 1 (MAPKAP1) is also named as MIP1 and SIN1. SIN1/MAPKAP1 is a major partner of mTORC2, acting as a scaffold and responsible for the substrate specificity of the mTOR catalytic subunit (PMID: 37877351). Besides as a component of MTORC2, MAPKAP1 also functions as MEKK2 and Ras-interacting protein and involves in regulating MEKK2 activation and RAS signaling (PMID: 31773361).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIN1/MAPKAP1 antibody 85702-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |