SIL1 Polyclonal antibody

SIL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24110-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

UNQ545/PRO836, ULG5, MSS, BiP-associated protein, BiP associated protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue, mouse colon tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

24110-1-AP targets SIL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 SIL1 fusion protein Ag21367 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
别名 UNQ545/PRO836, ULG5, MSS, BiP-associated protein, BiP associated protein
计算分子量 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC011568
基因名称 SIL1
Gene ID (NCBI) 64374
RRIDAB_2879434
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9H173
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIL1 antibody 24110-1-APDownload protocol
IHC protocol for SIL1 antibody 24110-1-APDownload protocol
IF protocol for SIL1 antibody 24110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Proteomic Analysis of Marinesco-Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss.

Authors - Francesca Potenza
mouseWB

Mol Biol Cell

Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion.

Authors - Viraj P Ichhaporia
mouseWB

Mol Neurobiol

Loss of SIL1 Affects Actin Dynamics and Leads to Abnormal Neural Migration

Authors - Yuanyuan Xu
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