• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SIL1 Polyclonal antibody

SIL1 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 24110-1-AP

Print datasheet

Synonyms

BAP, BiP associated protein, MSS, SIL1, ULG5

验证数据展示

  • WB analysis using 24110-1-AP

    WB analysis using 24110-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24110-1-AP (SIL1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24110-1-AP

    IF Staining of HepG2 using 24110-1-AP

    Immunofluorescent analysis of HepG2 cells using 24110-1-AP (SIL1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • IHC staining of human kidney using 24110-1-AP

    IHC staining of human kidney using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 24110-1-AP

    IHC staining of human kidney using 24110-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 24110-1-AP (SIL1 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman kidney tissue, mouse colon tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

The immunogen of 24110-1-AP is SIL1 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen SIL1 fusion protein Ag21367 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SIL1 homolog, endoplasmic reticulum chaperone (S. cerevisiae)
Synonyms BAP, BiP associated protein, MSS, SIL1, ULG5
Calculated Molecular Weight 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC011568
Gene Symbol SIL1
Gene ID (NCBI) 64374
RRIDAB_2879434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9H173
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIL1 antibody 24110-1-APDownload protocol
IHC protocol for SIL1 antibody 24110-1-APDownload protocol
IF protocol for SIL1 antibody 24110-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Int J Mol Sci

Proteomic Analysis of Marinesco-Sjogren Syndrome Fibroblasts Indicates Pro-Survival Metabolic Adaptation to SIL1 Loss.

Authors - Francesca Potenza
View Article
mouseWB

Mol Biol Cell

Sil1, a nucleotide exchange factor for BiP, is not required for antibody assembly or secretion.

Authors - Viraj P Ichhaporia
View Article