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- KD/KO Validated
SIGMAR1 Polyclonal antibody
SIGMAR1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
IHC, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, mouse kidney tissue, mouse colon tissue, rat colon tissue |
| Positive IHC detected in | human liver cancer tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15168-1-AP targets SIGMAR1 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC, CoIP |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Immunogen | SIGMAR1 fusion protein Ag7351 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | sigma non-opioid intracellular receptor 1 |
| Synonyms | hSigmaR1, OPRS1, SIG 1R, Sigma 1 type opioid receptor, Sigma1 receptor, Sigma1R, SIGMAR1, SR BP, SR BP1, SR31747 binding protein, SRBP |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC004899 |
| Gene Symbol | SIGMAR1 |
| Gene ID (NCBI) | 10280 |
| RRID | AB_2301712 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99720 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIGMAR1, also named as OPRS1, SRBP, SIG-1R and AAG8, belongs to the ERG2 family. It is an endoplasmic reticulum chaperone that binds a wide variety of ligands, including neurosteroids, psychostimulants, and dextrobenzomorphans. SIGMAR1 is ubiquitously expressed, and is enriched in motor neurons of the brain and spinal cord. It plays a role in calcium signaling through modulation together with ANK2 of the ITP3R-dependent calcium efflux at the endoplasmic reticulum. SIGMAR1 plays a role in several other cell functions including proliferation, survival and death. SDS-PAGE and Western blot analysis showed that SIGMAR1 has an apparent molecular mass of 28 kDa. SIGMAR1 has 5 isoforms with MW 21-27kDa and 12kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIGMAR1 antibody 15168-1-AP | Download protocol |
| IHC protocol for SIGMAR1 antibody 15168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models. | ||
Ann Neurol Hereditary Spastic Paraplegia-Linked REEP1 Modulates ER-Mitochondria Contacts. | ||
Proc Natl Acad Sci U S A DJ-1 regulates the integrity and function of ER-mitochondria association through interaction with IP3R3-Grp75-VDAC1. | ||
Schizophr Bull Allosteric Modulation of the Sigma-1 Receptor Elicits Antipsychotic-like Effects. | ||
Cell Mol Life Sci PACS2-TRPV1 axis is required for ER-mitochondrial tethering during ER stress and lung fibrosis. | ||
Int J Mol Sci Sigma-1 Receptor Is Critical for Mitochondrial Activity and Unfolded Protein Response in Larval Zebrafish.
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