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验证数据展示

  • WB analysis of HL-60 using 13377-1-AP (same clone as 13377-1-PBS)

    WB analysis of HL-60 using 13377-1-AP (same clone as 13377-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13377-1-AP (Siglec-9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

    IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

    IHC staining of human liver cancer using 13377-1-AP (same clone as 13377-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13377-1-AP (Siglec-9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

  • IF Staining of human tonsillitis using 13377-1-AP (same clone as 13377-1-PBS)

    IF Staining of human tonsillitis using 13377-1-AP (same clone as 13377-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using Siglec-9 antibody (13377-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13377-1-PBS in a different storage buffer formulation.

Siglec-9 Polyclonal antibody, PBS Only

Siglec-9 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 13377-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

SIGLEC9, CD329, Protein FOAP 9, Sialic acid-binding Ig-like lectin 9, Siglec 9

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

13377-1-PBS targets Siglec-9 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4201

Product name: Recombinant human SIGLEC9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 211-341 aa of BC035365

Sequence: LTCQVTFPGASVTTNKTVHLNVSYPPQNLTMTVFQGDGTVSTVLGNGSSLSLPEGQSLRLVCAVDAVDSNPPARLSLSWRGLTLCPSQPSNPGVLELPWVHLRDEAEFTCRAQNPLGSQQVYLNVSLQSKA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sialic acid binding Ig-like lectin 9
别名 SIGLEC9, CD329, Protein FOAP 9, Sialic acid-binding Ig-like lectin 9, Siglec 9
计算分子量 463 aa, 50 kDa
观测分子量 55-60 kDa
GenBank蛋白编号BC035365
基因名称 Siglec-9
Gene ID (NCBI) 27180
RRIDAB_2187293
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y336
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sialic acid binding Ig-like lectin 9 (Siglec-9), also known as CD329, is a member of the Siglec family of glycan-recognition proteins. Siglec-9 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains an N-terminal V-set domain and two C2-set domains, a transmembrane region, and an intracellular domain with an immunoreceptor tyrosine-based inhibitory motif (ITIM) and an ITIM-like motif (PMID: 10801862). It is expressed quite broadly among human blood leukocytes, including monocytes, neutrophils, B cells, NK cells, and minor subsets of T cells (PMID: 32322597). Siglec-9 functions as an inhibitory immune checkpoint and can be targeted to enhance therapeutic antitumor immunity (PMID: 34155121; 37460871).

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