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验证数据展示

  • WB analysis using 13964-1-AP

    WB analysis using 13964-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13964-1-AP (SIGLEC12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Siglec-12 Polyclonal antibody

Siglec-12 Polyclonal Antibody for WB, ELISA
Cat No. 13964-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, ELISA

别名

S2V, Siglec 12, Siglec L1, Siglec XII, SIGLEC12, Siglec-12, SIGLECL1, SLG

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse spleen tissue, rat spleen tissue, pig spleen tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13964-1-AP targets Siglec-12 in WB, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 Siglec-12 fusion protein Ag4939 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sialic acid binding Ig-like lectin 12
别名 S2V, Siglec 12, Siglec L1, Siglec XII, SIGLEC12, Siglec-12, SIGLECL1, SLG
计算分子量 595 aa, 65 kDa
观测分子量70 kDa
GenBank蛋白编号BC035809
基因名称 SIGLEC12
Gene ID (NCBI) 89858
RRIDAB_3669172
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PQ1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sialic acid-binding immunoglobulin-like lectins (SIGLECs) are a family of cell surface proteins belonging to the immunoglobulin superfamily that contain sialoside-binding N-terminal domains (PMID: 22749793). Sialic acid binding Ig-like lectin 12 (Siglec-12, also known as S2V, SLG, SIGLECL1, and Siglec-XII) is a member of the SIGLEC3-like subfamily of SIGLECs, which is highly expressed in the spleen, such as CD8+ cells, macrophages, neutrophils, and dendritic cells. It mediates sialic-acid-dependent binding to cells and could be an identification of therapeutic targets and prognostic biomarkers of sarcomas (PMID: 38182638).

实验方案

Product Specific Protocols
WB protocol for Siglec-12 antibody 13964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols