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SIDT1 Polyclonal antibody

SIDT1 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 55352-1-AP

Print datasheet

Synonyms

B830021E24Rik, SID 1, SID1, SIDT1

验证数据展示

  • WB analysis using 55352-1-AP

    WB analysis using 55352-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 55352-1-AP (SIDT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 55352-1-AP

    IHC staining of human breast cancer using 55352-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 55352-1-AP (SIDT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 55352-1-AP

    IF Staining of HepG2 using 55352-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using SIDT1 antibody (55352-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inBxPC-3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55352-1-AP targets SIDT1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SID1 transmembrane family, member 1
Synonyms B830021E24Rik, SID 1, SID1, SIDT1
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 95-110 kDa
GenBank Accession NumberNM_017699
Gene Symbol SIDT1
Gene ID (NCBI) 54847
RRIDAB_11182721
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NXL6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIDT1, also known as a human orthologue of SID-1, belongs to the SID family which is a highly conserved group of transmembrane channel-like proteins. As a transmembrane channel for intercellular communication, SIDT1 was found to facilitate small interfering RNA (siRNA) or miRNA uptake and also involved in cholesterol transport in human systems. SIDT1 mRNA and protein were mainly detected in the stomach and large intestine (including the cecum, colon and rectum) but not in the small intestine (especially the duodenum). Notably, SIDT1 was mainly located in gastric surface mucous cells (pit cells) but barely expressed in the parietal or chief cells. The SIDT1 gene encodes an 827 amino acid protein with a calculated molecular mass of 94 kDa. (PMID: 31632087, PMID: 32801357, PMID: 22174421)

实验方案

Product Specific Protocols
WB protocol for SIDT1 antibody 55352-1-APDownload protocol
IHC protocol for SIDT1 antibody 55352-1-APDownload protocol
IF protocol for SIDT1 antibody 55352-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Onco Targets Ther

Integrated Bioinformatics Data Analysis Reveals Prognostic Significance Of SIDT1 In Triple-Negative Breast Cancer.

Authors - Ya Wang
View Article