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SIAH2 Polyclonal antibody

SIAH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 12651-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Siah 2, RING-type E3 ubiquitin transferase SIAH2, hSiah2, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SIAH2 Ready-To-Use IHC Kit. SIAH2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12651-1-AP targets SIAH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 SIAH2 fusion protein Ag3342 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 seven in absentia homolog 2 (Drosophila)
别名 Siah 2, RING-type E3 ubiquitin transferase SIAH2, hSiah2, EC:2.3.2.27, E3 ubiquitin-protein ligase SIAH2
计算分子量 324 aa, 35 kDa
观测分子量 35-40 kDa
GenBank蛋白编号BC013082
基因名称 SIAH2
Gene ID (NCBI) 6478
RRIDAB_2877868
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43255
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mammalian Seven-in-absentia homolog 2 (SIAH2), is a RING finger type ubiquitin ligase with a catalytic RING domain on its N-terminus, followed by two zinc fingers and a C-terminal substrate binding domain. Siah is a mammalian homolog of Sina, a Drosophila protein functioning in eye development. Mammals express two isoforms, Siah1 and 2, and mice exhibit two Siah1 isotypes, 1a and 1b. SIAH2 indirectly regulates the level of HIF-1α by promoting degradation of PHDs by UPS. SIAH2, in turn, is positively regulated by hypoxia-dependent transcriptional and posttranscriptional mechanisms.

实验方案

Product Specific Protocols
WB protocol for SIAH2 antibody 12651-1-APDownload protocol
IHC protocol for SIAH2 antibody 12651-1-APDownload protocol
IF protocol for SIAH2 antibody 12651-1-APDownload protocol
IP protocol for SIAH2 antibody 12651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

Heat stress activates YAP/TAZ to induce the heat shock transcriptome.

Authors - Min Luo
  • KD Validated
humanWB

Nucleic Acids Res

P-TEFb is degraded by Siah1/2 in quiescent cells.

Authors - Fang Huang
  • KD Validated
humanWB

J Exp Clin Cancer Res

PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer

Authors - Liang Xu
humanWB,IP

Int J Biol Sci

Cold atmospheric plasma potentiates ferroptosis via EGFR(Y1068)-mediated dual axes on GPX4 among triple negative breast cancer cells

Authors - Xiaofeng Dai

Cell Death Dis

PIWIL2 suppresses Siah2-mediated degradation of HDAC3 and facilitates CK2α-mediated HDAC3 phosphorylation.

Authors - Yingying Zhang
mouseIF,WB

Int Immunopharmacol

Methylene blue targets PHD3 expression in murine microglia to mitigate lipopolysaccharide-induced neuroinflammation and neurocognitive impairments

Authors - Guoyao Ou
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