×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of HeLa using 13886-1-AP (same clone as 13886-1-PBS)

    WB analysis of HeLa using 13886-1-AP (same clone as 13886-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 13886-1-AP (SIAH1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

    IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIAH1 antibody included
  • IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

    IHC staining of mouse testis using 13886-1-AP (same clone as 13886-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13886-1-AP (SIAH1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13886-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SIAH1 antibody included
  • Featured Product
  • KD/KO Validated

SIAH1 Polyclonal antibody, PBS Only

SIAH1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 13886-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy SIAH1 Ready-To-Use IHC Kit. SIAH1 primary antibody included.

产品信息

13886-1-PBS targets SIAH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4336

Product name: Recombinant human SIAH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-282 aa of BC035562

Sequence: MSRQTATALPTGTSKCPPSQRVPALTGTTASNNDLASLFECPVCFDYVLPPILQCQSGHLVCSNCRPKLTCCPTCRGPLGSIRNLAMEKVANSVLFPCKYASSGCEITLPHTEKADHEELCEFRPYSCPCPGASCKWQGSLDAVMPHLMHQHKSITTLQGEDIVFLATDINLPGAVDWVMMQSCFGFHFMLVLEKQEKYDGHQQFFAIVQLIGTRKQAENFAYRLELNGHRRRLTWEATPRSIHEGIATAIMNSDCLVFDTSIAQLFAENGNLGINVTISMC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 seven in absentia homolog 1 (Drosophila)
别名
计算分子量 282 aa, 31 kDa
观测分子量 60 kDa
GenBank蛋白编号BC035562
基因名称 SIAH1
Gene ID (NCBI) 6477
RRIDAB_10643374
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUQ4
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIAH1, also named as HUMSIAH, Siah-1a and Siah-1S(21kd), belongs to the SINA (Seven in absentia) family. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. SIAH1 can form heterodimer or homodimer such as Siah-1*Siah-1(70 or 62kd), Siah-1*Siah-1S(42kd) and Siah-1S*Siah-1S(51-56kd). Importantly, results from in vitro soft agar assay demonstrated that Siah-1S displays a promotion effect on cells tumorigenicity. (PMID:17420721)

||
New chat

Able™

正在加载，请稍候...