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验证数据展示

  • WB analysis using 66522-1-Ig

    WB analysis using 66522-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig colon using 66522-1-Ig

    WB analysis of pig colon using 66522-1-Ig

    pig colon tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 66522-1-Ig

    IF Staining of PC-3 using 66522-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SIAE antibody (66522-1-Ig, Clone: 1G5F7 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

SIAE Monoclonal antibody

SIAE Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66522-1-Ig

产品说明书

CloneNo. 1G5F7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inpig liver tissue, pig colon tissue, rat colon tissue, mouse liver tissue
Positive IF/ICC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66522-1-Ig targets SIAE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag14624

Product name: Recombinant human SIAE protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC068450

Sequence: MVAPGLVLGLVLPLILWADRSAGIGFRFASYINNDMVLQKEPAGAVIWGFGTPGATVTVTLRQGQETIMKKVTSVKAHSDTWMVVLDPMKPGGPFEVMAQQTLEKINFTLRVHDVLFGDVWLCSGQSNMQMTVLQIFNATRELSNTAAYQSVRILSVSPIQAEQELEDLVAVDLQWSKPTSENLGHGYFKYMSAVCWLFGRHLYDTLQYPIGLIASSWGGTPIEAWSSGRSLKACGVPKQGSIPYDSVTGPSKHSVLWNAMIHPLCNMTLKGVVWYQGESNINYNTDLYNCTFPALIEDWRETFHRGSQGQTERFFPFGLVQLSSDLSKKSSDDGFPQIRWHQTADFGYV

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 sialic acid acetylesterase
别名 Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53
计算分子量 58 kDa
观测分子量 70 kDa
GenBank蛋白编号BC068450
基因名称 SIAE
Gene ID (NCBI) 54414
RRIDAB_2881885
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9HAT2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for SIAE antibody 66522-1-IgDownload protocol
WB protocol for SIAE antibody 66522-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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