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验证数据展示

  • IHC staining of mouse testis using 31712-1-AP

    IHC staining of mouse testis using 31712-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31712-1-AP (SHROOM4 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 31712-1-AP

    IHC staining of human placenta using 31712-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 31712-1-AP (SHROOM4 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HUVEC using 31712-1-AP

    IF Staining of HUVEC using 31712-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using SHROOM4 antibody (31712-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SHROOM4 Polyclonal antibody

SHROOM4 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 31712-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

KIAA1202, Protein Shroom4, shroom family member 4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman placenta tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31712-1-AP targets SHROOM4 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag36423

Product name: Recombinant human SHROOM4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1170-1320 aa of BC151240

Sequence: GSCALNPEEVLEQPQPLSFGHLEGSRQGSQSVPAEQESFALHSSDFLPPIRGHLGSQPEQAQPPCYYGIGGLWRTSGQEATESAKQEFQHFSPPSGAPGIPTSYSAYYNISVAKAELLNKLKDQPEMAEIGLGEEEVDHELAQKKIQLIES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 shroom family member 4
别名 KIAA1202, Protein Shroom4, shroom family member 4
GenBank蛋白编号BC151240
基因名称 SHROOM4
Gene ID (NCBI) 57477
RRIDAB_3742464
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9ULL8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Shroom-related gene SHROOM4 (formerly called KIAA1202) has also been implicated in neural development, as mutations in this gene are associated with human X-linked mental retardation. SHROOM4 is expressed in a wide range of tissue types during mouse development, including the vascular endothelium of the lung and the polarized epithelium of the neural tube and kidney.

实验方案

Product Specific Protocols
IF protocol for SHROOM4 antibody 31712-1-APDownload protocol
IHC protocol for SHROOM4 antibody 31712-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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