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验证数据展示

  • WB analysis of mouse testis using 21955-1-AP

    WB analysis of mouse testis using 21955-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21955-1-AP (SHPRH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 21955-1-AP

    IP experiment of mouse testis using 21955-1-AP

    IP result of anti-SHPRH (IP:21955-1-AP, 4ug; Detection:21955-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • IHC staining of human colon using 21955-1-AP

    IHC staining of human colon using 21955-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21955-1-AP (SHPRH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon using 21955-1-AP

    IHC staining of human colon using 21955-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21955-1-AP (SHPRH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SHPRH Polyclonal antibody

SHPRH Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 21955-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, IHC, ELISA

别名

KIAA2023, SHPRH

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21955-1-AP targets SHPRH in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 SHPRH fusion protein Ag16635 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SNF2 histone linker PHD RING helicase
别名 KIAA2023, SHPRH
计算分子量 1683 aa, 193 kDa
观测分子量 200 kDa
GenBank蛋白编号BC117686
基因名称 SHPRH
Gene ID (NCBI) 257218
RRIDAB_2878954
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ149N8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHPRH antibody 21955-1-APDownload protocol
IHC protocol for SHPRH antibody 21955-1-APDownload protocol
IP protocol for SHPRH antibody 21955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols