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验证数据展示

  • WB analysis using 12441-1-AP

    WB analysis using 12441-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12441-1-AP (SHPK antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 12441-1-AP

    IHC staining of human prostate cancer using 12441-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12441-1-AP (SHPK antibody) at dilution of 1:50 (under 10x lens).

SHPK Polyclonal antibody

SHPK Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12441-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CARKL, sedoheptulokinase, SHK, SHPK

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-231 cells, RAW 264.7 cells, mouse heart tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12441-1-AP targets SHPK in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3150

Product name: Recombinant human SHPK protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 131-478 aa of BC020543

Sequence: RCSSEFLASLPQPKSHLSVATGFGCATIFWLLKYRPEFLKSYDAAGTIHDYVVAMLCGLPRPLMSDQNAASWGYFNTQSQSWNVETLRSSGFPVHLLPDIAEPGSVAGRTSHMWFEIPKGTQVGVALGDLQASVYSCMAQRTDAVLNISTSVQLAASMPSGFQPAQTPDPTAPVAYFPYFNRTYLGVAASLNGGNVLATFVHMLVQWMADLGLEVEESTVYSRMIQAAVQQRDTHLTITPTVLGERHLPDQLASVTRISSSDLSLGHVTRALCRGIVQNLHSMLPIQQLQEWGVERVMGSGSALSRNDVLKQEVQRAFPLPMSFGQDVDAAVGAALVMLRRHLNQKES

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sedoheptulokinase
别名 CARKL, sedoheptulokinase, SHK, SHPK
计算分子量 478 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC020543
基因名称 SHPK
Gene ID (NCBI) 23729
RRIDAB_2188566
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHJ6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHPK, also named CARKL, is a carbohydrate kinase involved in the phosphorylation of sugars as they enter a cell, inhibiting return across the cell membrane (PubMed: 10673275). SHPK is a sedoheptulose kinase of the pentose phosphate pathway. It acts as a modulator of macrophage activation through control of glucose metabolism (PMID: 22682222).

实验方案

Product Specific Protocols
WB protocol for SHPK antibody 12441-1-APDownload protocol
IHC protocol for SHPK antibody 12441-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols