验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, fetal human brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
25117-1-AP targets SHOX in WB, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | SHOX fusion protein Ag18930 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | short stature homeobox |
别名 | GCFX, PHOG, short stature homeobox, Short stature homeobox protein, SHOX, SHOXY, SS |
计算分子量 | 292 aa, 32 kDa |
观测分子量 | 25-32 kDa |
GenBank蛋白编号 | BC148783 |
基因名称 | SHOX |
Gene ID (NCBI) | 6473 |
RRID | AB_2879905 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O15266 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Short stature homeobox protein (SHOX), also named as GCFX, is a 292 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. SHOX. SHOX controls fundamental aspects of growth and development. SHOX undergoes splicing resulting in two isoforms, SHOXA and SHOXB. SHOXA is a 32 kDa protein and is expressed in skeletal muscle, placental, pancreas, heart and bone marrow fibroblast and, to a lesser extent, in kidney and lung. SHOXB is 25 kDa protein and is highly expressed in osteogenic cells, but not expressed in brain, kidney, liver or lung.
实验方案
Product Specific Protocols | |
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WB protocol for SHOX antibody 25117-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |