验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, fetal human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
25117-1-AP targets SHOX in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18930 Product name: Recombinant human SHOX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 17-110 aa of BC148783 Sequence: KDGNGGGGGGGGKKDSITYREVLESGLARSRELGTSDSSLQDITEGGGHCPVHLFKDHVDNDKEKLKEFGTARVAEGIYECKEKREDVKSEDED 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | short stature homeobox |
| 别名 | GCFX, PHOG, short stature homeobox, Short stature homeobox protein, SHOX, SHOXY, SS |
| 计算分子量 | 292 aa, 32 kDa |
| 观测分子量 | 25-32 kDa |
| GenBank蛋白编号 | BC148783 |
| 基因名称 | SHOX |
| Gene ID (NCBI) | 6473 |
| RRID | AB_2879905 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15266 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Short stature homeobox protein (SHOX), also named as GCFX, is a 292 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the paired homeobox family. SHOX. SHOX controls fundamental aspects of growth and development. SHOX undergoes splicing resulting in two isoforms, SHOXA and SHOXB. SHOXA is a 32 kDa protein and is expressed in skeletal muscle, placental, pancreas, heart and bone marrow fibroblast and, to a lesser extent, in kidney and lung. SHOXB is 25 kDa protein and is highly expressed in osteogenic cells, but not expressed in brain, kidney, liver or lung.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SHOX antibody 25117-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
