验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human stomach cancer tissue, human placenta tissue, mouse testis tissue, mouse brain tissue, rat stomach tissue, rat brain tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy SHKBP1 Ready-To-Use IHC Kit. SHKBP1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12703-1-AP targets SHKBP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag3386 Product name: Recombinant human SHKBP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 358-707 aa of BC022855 Sequence: MKDNDLLVSELYRDPAEDGVTALSVYLTPKTSDSGNWIEIAYGTSSGGVRVIVQHPETVGSGPQLFQTFTVHRSPVTKIMLSEKHLISVCADNNHVRTWSVTRFRGMISTQPGSTPLASFKILALESADGHGGCSAGNDIGPYGERDDQLVFIQKVVPSASQLFVRLSSTGQRVCSVRSVDGSPTTAFTVLECEGSRRLGSRPRRYLLTGQANGSLAMWDLTTAMDGLGQAPAGGLTEQELMEQLEHCELAPPAPSAPSWGCLPSPSPRISLTSLHSASSNTSLSGHRGSPSPPQAEARRRGGGSFVERCQELVRSGPDLRRPPTPAPWPSSGLGTPLTPPKMKLNETSF 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SH3KBP1 binding protein 1 |
| 别名 | PP203, SB1, SETA-binding protein 1, SH3KBP1 binding protein 1, SH3KBP1-binding protein 1 |
| 计算分子量 | 707 aa, 76 kDa |
| 观测分子量 | 76 kDa |
| GenBank蛋白编号 | BC022855 |
| 基因名称 | SHKBP1 |
| Gene ID (NCBI) | 92799 |
| RRID | AB_10637860 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8TBC3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SHKBP1 antibody 12703-1-AP | Download protocol |
| IHC protocol for SHKBP1 antibody 12703-1-AP | Download protocol |
| IF protocol for SHKBP1 antibody 12703-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
