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Species Reactivity
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验证数据展示

  • WB analysis of A431 using 12703-1-AP

    WB analysis of A431 using 12703-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12703-1-AP (SHKBP1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 12703-1-AP

    IF Staining of A549 using 12703-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using SHKBP1 antibody (12703-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SHKBP1 Polyclonal antibody

SHKBP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 12703-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

SH3KBP1-binding protein 1, SH3KBP1 binding protein 1, SETA-binding protein 1, SB1, PP203

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy SHKBP1 Ready-To-Use IHC Kit. SHKBP1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12703-1-AP targets SHKBP1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SHKBP1 fusion protein Ag3386 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3KBP1 binding protein 1
别名 SH3KBP1-binding protein 1, SH3KBP1 binding protein 1, SETA-binding protein 1, SB1, PP203
计算分子量 707 aa, 76 kDa
观测分子量 76 kDa
GenBank蛋白编号BC022855
基因名称 SHKBP1
Gene ID (NCBI) 92799
RRIDAB_10637860
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TBC3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHKBP1 antibody 12703-1-APDownload protocol
IF protocol for SHKBP1 antibody 12703-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols