验证数据展示
经过测试的应用
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30240-1-AP targets SHISA8 in IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | Human |
| 免疫原 |
CatNo: Ag31420 Product name: Recombinant human SHISA8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 160-300 aa of NM_001353438 Sequence: RLGLERAHSPRARRTVTRALTELLKQPGPQEPLPPTLGPPLGGCVQVQMGDGLPRGSPHNSADKKRLNNAPRGSAAPGPPRGPRLQGGGSLTLQPDYAKYATFKAAALKAAEAAPRDFCQRFPALEPSPRQPPARAPRPSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | transmembrane protein 46-like |
| 别名 | C22-CTA-250D10.9, C22orf17, LOC440829, Putative protein shisa 8, SHISA8, transmembrane protein 46 like |
| 计算分子量 | 42KD |
| GenBank蛋白编号 | NM_001353438 |
| 基因名称 | SHISA8 |
| Gene ID (NCBI) | 440829 |
| RRID | AB_3086274 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | B8ZZ34 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The least studied AMPAR-interacting member of the Shisa protein family is SHISA8 (CKAMP39). In HEK293 cells, SHISA8 significantly affected AMPAR-mediated current kinetics, such as current amplitude, as well as AMPAR desensitization and recovery from desensitization (PMID:33892364).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SHISA8 antibody 30240-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
