验证数据展示
经过测试的应用
Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30240-1-AP targets SHISA8 in IHC, ELISA applications and shows reactivity with Human samples.
经测试应用 | IHC, ELISA Application Description |
经测试反应性 | Human |
免疫原 | SHISA8 fusion protein Ag31420 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | transmembrane protein 46-like |
别名 | C22-CTA-250D10.9, C22orf17, LOC440829, Putative protein shisa 8, SHISA8, transmembrane protein 46 like |
计算分子量 | 42KD |
GenBank蛋白编号 | NM_001353438 |
基因名称 | SHISA8 |
Gene ID (NCBI) | 440829 |
RRID | AB_3086274 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | B8ZZ34 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The least studied AMPAR-interacting member of the Shisa protein family is SHISA8 (CKAMP39). In HEK293 cells, SHISA8 significantly affected AMPAR-mediated current kinetics, such as current amplitude, as well as AMPAR desensitization and recovery from desensitization (PMID:33892364).
实验方案
Product Specific Protocols | |
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IHC protocol for SHISA8 antibody 30240-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |