验证数据展示
经过测试的应用
| Positive IHC detected in | human skin cancer tissue, human cervical cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy DSS1 Ready-To-Use IHC Kit. DSS1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13639-1-AP targets DSS1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag4573 Product name: Recombinant human SHFM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-70 aa of BC032782 Sequence: MSEKKQPVDLGLLEEDDEFEEFPAEDWAGLDEDEDAHVWEDNWDDDNVEDDFSNQLRAELEKHGYKMETS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | split hand/foot malformation (ectrodactyly) type 1 |
| 别名 | DSS1, ECD, SEM1, SHFD1, Shfdg1, SHFM1, SHSF1 |
| 计算分子量 | 70 aa, 8 kDa |
| GenBank蛋白编号 | BC032782 |
| 基因名称 | DSS1 |
| Gene ID (NCBI) | 7979 |
| RRID | AB_2254633 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P60896 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for DSS1 antibody 13639-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature BRCA2 prevents R-loop accumulation and associates with TREX-2 mRNA export factor PCID2.
| ||
EMBO J The proteasomal de-ubiquitinating enzyme POH1 promotes the double-strand DNA break response. | ||
J Med Genet Absent expression of the osteoblast-specific maternally imprinted genes, DLX5 and DLX6, causes split hand/split foot malformation type I. | ||
Lab Invest Increased chemosensitivity via BRCA2-independent DNA damage in DSS1- and PCID2-depleted breast carcinomas.
| ||
BMC Cancer Breast cancers with high DSS1 expression that potentially maintains BRCA2 stability have poor prognosis in the relapse-free survival. | ||
Protein Cell DSSylation, a novel protein modification targets proteins induced by oxidative stress, and facilitates their degradation in cells. |
