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验证数据展示

  • IHC staining of human breast cancer using 22902-1-AP

    IHC staining of human breast cancer using 22902-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22902-1-AP (SHE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of mouse testis using 22902-1-AP

    WB analysis of mouse testis using 22902-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 22902-1-AP (SHE Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 22902-1-AP

    IF Staining of HepG2 using 22902-1-AP

    Immunofluorescent analysis of HepG2 cells using 22902-1-AP (SHE antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

SHE Polyclonal antibody

SHE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 22902-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

RP11 350G8.8, SHE

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22902-1-AP targets SHE in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Src homology 2 domain containing E
别名 RP11 350G8.8, SHE
计算分子量 54 kDa
观测分子量 55 kDa
GenBank蛋白编号NM_001010846
基因名称 SHE
Gene ID (NCBI) 126669
RRIDAB_2879185
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ5VZ18
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHE, also known as SH2 domain-containing adapter protein E, is a 495 amino acid protein whose molecular weight is 54 kDa. The function of SHE remains unknown. This antibody specifically recognises the 54 kDa protein.

实验方案

Product Specific Protocols
WB protocol for SHE antibody 22902-1-APDownload protocol
IHC protocol for SHE antibody 22902-1-APDownload protocol
IF protocol for SHE antibody 22902-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols