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SHD Polyclonal antibody

SHD Polyclonal Antibody for IF/ICC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, WB,ELISA

Conjugate

Unconjugated

Cat No : 20814-1-AP

Print datasheet

Synonyms

SHD

验证数据展示

  • WB analysis of mouse brain using 20814-1-AP

    WB analysis of mouse brain using 20814-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20814-1-AP (SHD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of Neuro-2a using 20814-1-AP

    IF Staining of Neuro-2a using 20814-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed Neuro-2a cells using 20814-1-AP (SHD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, Neuro-2a cells, NIH/3T3 cells, SMMC-7721 cells
Positive IF detected inNeuro-2a cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20814-1-AP is SHD Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB,ELISA
Tested Reactivity human, mouse
Immunogen SHD fusion protein Ag14794 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name Src homology 2 domain containing transforming protein D
Synonyms SHD
Calculated Molecular Weight 340 aa, 38 kDa
Observed Molecular Weight 40-50 kDa
GenBank Accession NumberBC007206
Gene Symbol SHD
Gene ID (NCBI) 56961
RRIDAB_2878745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96IW2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHD antibody 20814-1-APDownload protocol
IF protocol for SHD antibody 20814-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols