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SHC3 Polyclonal antibody

SHC3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12436-1-AP

Print datasheet

Synonyms

N Shc, Neuronal Shc, NSHC, Protein Rai, RAI, SH2 domain protein C3, SHC transforming protein 3, SHC transforming protein C, SHC3, SHCC

验证数据展示

  • WB analysis using 12436-1-AP

    WB analysis using 12436-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12436-1-AP (SHC3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 12436-1-AP

    IHC staining of mouse pancreas using 12436-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 12436-1-AP

    IHC staining of mouse pancreas using 12436-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 12436-1-AP (SHC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of BxPC-3 using 12436-1-AP

    IP experiment of BxPC-3 using 12436-1-AP

    IP result of anti-SHC3 (IP:12436-1-AP, 4ug; Detection:12436-1-AP 1:300) with BxPC-3 cells lysate 2800ug.

经过测试的应用

Positive WB detected inBxPC-3 cells
Positive IP detected inBxPC-3 cells
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12436-1-AP targets SHC3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen SHC3 fusion protein Ag3115 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SHC (Src homology 2 domain containing) transforming protein 3
Synonyms N Shc, Neuronal Shc, NSHC, Protein Rai, RAI, SH2 domain protein C3, SHC transforming protein 3, SHC transforming protein C, SHC3, SHCC
Calculated Molecular Weight 594 aa, 64 kDa
Observed Molecular Weight 50-52 kDa
GenBank Accession NumberBC026314
Gene Symbol SHC3
Gene ID (NCBI) 53358
RRIDAB_2301798
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92529
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SHC3 antibody 12436-1-APDownload protocol
IHC protocol for SHC3 antibody 12436-1-APDownload protocol
IP protocol for SHC3 antibody 12436-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Lysate microarrays enable high-throughput, quantitative investigations of cellular signaling.

Authors - Mark Sevecka
View Article
mouseIHC

J Hepatocell Carcinoma

The Long Non-Coding RNA AC006329.1 Facilitates Hepatocellular Carcinoma Progression and Metastasis by Regulating miR-127-5p/SHC3/ERK Axis

Authors - Xiang Xu Kong
View Article