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验证数据展示

  • WB analysis using 18200-1-AP (same clone as 18200-1-PBS)

    WB analysis using 18200-1-AP (same clone as 18200-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 18200-1-AP (SHBG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18200-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18200-1-AP (same clone as 18200-1-PBS)

    IHC staining of human liver using 18200-1-AP (same clone as 18200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18200-1-AP (SHBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18200-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 18200-1-AP (same clone as 18200-1-PBS)

    IHC staining of mouse testis using 18200-1-AP (same clone as 18200-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18200-1-AP (SHBG antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18200-1-PBS in a different storage buffer formulation.

SHBG Polyclonal antibody, PBS Only

SHBG Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 18200-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

Testosterone-estradiol-binding globulin, Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

18200-1-PBS targets SHBG in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag12683

Product name: Recombinant human SHBG protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 30-402 aa of BC101785

Sequence: LRPVLPTQSAHDPPAVHLSNGPGQEPIAVMTFDLTKITKTSSSFEVRTWDPEGVIFYGDTNPKDDWFMLGLRDGRPEIQLHNHWAQLTVGAGPRLDDGRWHQVEVKMEGDSVLLEVDGEEVLRLRQVSGPLTSKRHPIMRIALGGLLFPASNLRLPLVPALDGCLRRDSWLDKQAEISASAPTSLRSCDVESNPGIFLPPGTQAEFNLRDIPQPHAEPWAFSLDLGLKQAAGSGHLLALGTPENPSWLSLHLQDQKVVLSSGSGPGLDLPLVLGLPLQLKLSMSRVVLSQGSKMKALALPPLGLAPLLNLWAKPQGRLFLGALPGEDSSTSFCLNGLWAQGQRLDVDQALNRSHEIWTHSCPQSPGNGTDASH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sex hormone-binding globulin
别名 Testosterone-estradiol-binding globulin, Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin
计算分子量 402 aa, 44 kDa
观测分子量 42-44 kDa
GenBank蛋白编号BC101785
基因名称 SHBG
Gene ID (NCBI) 6462
RRIDAB_2878517
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04278
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

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