验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, L02 cells, C2C12 cells, H9C2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31967-1-AP targets SHB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | SHB fusion protein Ag35936 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Src homology 2 domain containing adaptor protein B |
| 别名 | SH2 domain-containing adapter protein B |
| 计算分子量 | 55 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC136581 |
| 基因名称 | SHB |
| Gene ID (NCBI) | 6461 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q15464 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SH2 domain-containing adapter protein B (SHB) is an adaptor protein that operates downstream of tyrosine kinases. It is crucial in various cellular responses, including cell survival, differentiation, and proliferation (PMID: 29792386). SHB is involved in the modulation of focal adhesion kinase (FAK) signaling, which is important for regulating cell cycle progression in hematopoietic stem cells (PMID: 23528453). The protein has also been implicated in the progression of myeloproliferative disorders by affecting the activity of FAK and other signaling intermediates (PMID: 24952416).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SHB antibody 31967-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |