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验证数据展示

  • IP experiment of mouse brain using 27272-1-AP

    IP experiment of mouse brain using 27272-1-AP

    IP result of anti-SHANK2 (IP:27272-1-AP, 4ug; Detection:27272-1-AP 1:1000) with mouse brain tissue lysate 1600 ug.

  • WB analysis using 27272-1-AP

    WB analysis using 27272-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27272-1-AP (SHANK2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

SHANK2 Polyclonal antibody

SHANK2 Polyclonal Antibody for WB, IP, ELISA
Cat No. 27272-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

别名

SHANK, ProSAP1, KIAA1022, CTTNBP1, CORTBP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27272-1-AP targets SHANK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SHANK2 fusion protein Ag26101 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 and multiple ankyrin repeat domains 2
别名 SHANK, ProSAP1, KIAA1022, CTTNBP1, CORTBP1
计算分子量 159 kDa
观测分子量 158-200 kDa
GenBank蛋白编号NM_012309
基因名称 SHANK2
Gene ID (NCBI) 22941
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPX8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3 and multiple ankyrin repeat domains protein 2 (SHANK2) is also named as CORTBP1, KIAA1022, PROSAP1. SHANK2 code a scaffolding protein located at the postsynaptic membrane of glutamatergic neurons (PMID: 32987185). SHANK2 encodes for a postsynaptic scaffolding protein at glutamatergic synapses in the brain, essential for proper synapse formation, development and plasticity (PMID: 11283303, PMID: 12065602). As SHANK2 directly interacts with IRSp53 (insulin receptor substrate p53), it may be involved in insulin signaling in the brain, making this pathway susceptible to SHANK2 mutations (PMID: 33483523). In the SFARI gene database, SHANK2 is categorized as a high confidence autism risk gene. In addition, SHANK2 has been linked to the pathology of neuropsychiatric (schizophrenia, bipolar disorder) and neurodegenerative disorders (PMID: 33483523).

实验方案

Product Specific Protocols
WB protocol for SHANK2 antibody 27272-1-APDownload protocol
IP protocol for SHANK2 antibody 27272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols