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验证数据展示

  • IHC staining of human colon cancer using 13306-1-AP

    IHC staining of human colon cancer using 13306-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13306-1-AP (SH3RF2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 13306-1-AP

    WB analysis using 13306-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13306-1-AP (SH3RF2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 13306-1-AP

    IHC staining of human colon cancer using 13306-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13306-1-AP (SH3RF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Caco-2 using 13306-1-AP

    IF Staining of Caco-2 using 13306-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SH3RF2 antibody (13306-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SH3RF2 Polyclonal antibody

SH3RF2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 13306-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

POSH-eliminating RING protein, POSH3, HEPP1, Heart protein phosphatase 1-binding protein, EC:2.3.2.27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inCaco-2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13306-1-AP targets SH3RF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SH3RF2 fusion protein Ag4127 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 domain containing ring finger 2
别名 POSH-eliminating RING protein, POSH3, HEPP1, Heart protein phosphatase 1-binding protein, EC:2.3.2.27
计算分子量 729 aa, 79 kDa
GenBank蛋白编号BC031650
基因名称 SH3RF2
Gene ID (NCBI) 153769
RRIDAB_2918032
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3RF2 antibody 13306-1-APDownload protocol
IHC protocol for SH3RF2 antibody 13306-1-APDownload protocol
IF protocol for SH3RF2 antibody 13306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols