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验证数据展示

  • WB analysis using 88135-1-RR (same clone as 88135-1-PBS)

    WB analysis using 88135-1-RR (same clone as 88135-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88135-1-RR (SH3RF1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88135-1-PBS in a different storage buffer formulation.

  • WB analysis using 88135-1-RR (same clone as 88135-1-PBS)

    WB analysis using 88135-1-RR (same clone as 88135-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88135-1-RR (SH3RF1/POSH antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88135-1-PBS in a different storage buffer formulation.

SH3RF1/POSH Recombinant monoclonal antibody, PBS Only

SH3RF1/POSH Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 88135-1-PBS

产品说明书

CloneNo. 260354A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

POSH, SH3RF1, E3 ubiquitin-protein ligase SH3RF1, EC:2.3.2.27, KIAA1494

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy SH3RF1/POSH Ready-To-Use IHC Kit. SH3RF1/POSH primary antibody included.

产品信息

88135-1-PBS targets SH3RF1/POSH in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6243

Product name: Recombinant human SH3RF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 696-1056 aa of BC053671

Sequence: VTMVSPSTAGGPAQKLQGNGVAGSPSVVPAAVVSAAHIQTSPQAKVLLHMTGQMTVNQARNAVRTVAAHNQERPTAAVTPIQVQNAAGLSPASVGLSHHSLASPQPAPLMPGSATHTAAISISRASAPLACAAAAPLTSPSITSASLEAEPSGRIVTVLPGLPTSPDSASSACGNSSATKPDKDSKKEKKGLLKLLSGASTKRKPRVSPPASPTLEVELGSAELPLQGAVGPELPPGGGHGRAGSCPVDGDGPVTTAVAGAALAQDAFHRKASSLDSAVPIAPPPRQACSSLGPVLNESRPVVCERHRVVVSYPPQSEAELELKEGDIVFVHKKREDGWFKGTLQRNGKTGLFPGSFVENI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SH3 domain containing ring finger 1
别名 POSH, SH3RF1, E3 ubiquitin-protein ligase SH3RF1, EC:2.3.2.27, KIAA1494
计算分子量 93 kDa
观测分子量93-100 kDa
GenBank蛋白编号BC053671
基因名称 SH3RF1/POSH
Gene ID (NCBI) 57630
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ7Z6J0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POSH (plenty of SH3 domains) is a multidomain GTP-Rac1-interacting protein that acts as a scaffold for JNK activation, leading to apoptosis in neuronal and non-neuronal cells. It consists a RING-finger domain that possesses ubiquitin-ligase activity and four SH3 domains.

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