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验证数据展示

  • WB analysis using 27446-1-AP

    WB analysis using 27446-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27446-1-AP (SH3BP5L antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 27446-1-AP

    IHC staining of mouse cerebellum using 27446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27446-1-AP (SH3BP5L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse cerebellum using 27446-1-AP

    IHC staining of mouse cerebellum using 27446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 27446-1-AP (SH3BP5L antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SH3BP5L Polyclonal antibody

SH3BP5L Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27446-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KIAA1720, SH3BP 5 like

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected in769-P cells, PC-3 cells, RKO cells, THP-1 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27446-1-AP targets SH3BP5L in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag23990

Product name: Recombinant human SH3BP5L protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 231-393 aa of BC017254

Sequence: QFSQILEEHKAKVTELEQQVAQAKTRYSVALRNLEQISEQIHARRRGGLPPHPLGPRRSSPVGAEAGPEDMEDGDSGIEGAEGAGLEEGSSLGPGPAPDTDTLSLLSLRTVASDLQKCDSVEHLRGLSDHVSLDGQELGTRSGGRRGSDGGARGGRHQRSVSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3-binding domain protein 5-like
别名 KIAA1720, SH3BP 5 like
观测分子量65-70 kDa
GenBank蛋白编号BC017254
基因名称 SH3BP5L
Gene ID (NCBI) 80851
RRIDAB_3742231
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L8J4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3BP5L (SH3 Binding Domain Protein 5 Like) is a protein-coding gene that encodes a protein with a conserved N-terminus and functions as a guanine nucleotide exchange factor (GEF) for Rab11 GTPases.

实验方案

Product Specific Protocols
IHC protocol for SH3BP5L antibody 27446-1-APDownload protocol
WB protocol for SH3BP5L antibody 27446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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