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SH3BGRL3 Polyclonal antibody

SH3BGRL3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12210-1-AP

Print datasheet

Synonyms

SH3 domain binding protein 1, SH3BGRL3, SH3BP 1, TIP B1

验证数据展示

  • WB analysis using 12210-1-AP

    WB analysis using 12210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 12210-1-AP

    IHC staining of human stomach cancer using 12210-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 12210-1-AP

    IHC staining of human stomach cancer using 12210-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inPC-3 cells, U-251 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12210-1-AP targets SH3BGRL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen SH3BGRL3 fusion protein Ag2857 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3 domain binding glutamic acid-rich protein like 3
Synonyms SH3 domain binding protein 1, SH3BGRL3, SH3BP 1, TIP B1
Calculated Molecular Weight 93 aa, 10 kDa
Observed Molecular Weight10 kDa
GenBank Accession NumberBC030135
Gene Symbol SH3BGRL3
Gene ID (NCBI) 83442
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H299
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein, could act as a modulator of glutaredoxin biological activity. It may play a role in cytoskeleton organization (PMID: 34380438). SH3BGRL3 acts as a potential prognostic biomarker for urothelial carcinoma and a novel binding partner of epidermal growth factor receptor (PMID: 26286913).

实验方案

Product Specific Protocols
WB protocol for SH3BGRL3 antibody 12210-1-APDownload protocol
IHC protocol for SH3BGRL3 antibody 12210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Chem Biol

Citrullination/Methylation Crosstalk on Histone H3 Regulates ER-Target Gene Transcription.

Authors - Kathleen W Clancy
View Article