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验证数据展示

  • WB analysis using 12210-1-AP

    WB analysis using 12210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 12210-1-AP

    IHC staining of human stomach cancer using 12210-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 12210-1-AP

    IHC staining of human stomach cancer using 12210-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SH3BGRL3 Polyclonal antibody

SH3BGRL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12210-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SH3 domain binding protein 1, SH3BGRL3, SH3BP 1, TIP B1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells, U-251 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12210-1-AP targets SH3BGRL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原

CatNo: Ag2857

Product name: Recombinant human SH3BGRL3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-93 aa of BC030135

Sequence: MSGLRVYSTSVTGSREIKSQQSEVTRILDGKRIQYQLVDISQDNALRDEMRALAGNPKATPPQIVNGDQYCGDYELFVEAVEQNTLQEFLKLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 domain binding glutamic acid-rich protein like 3
别名 SH3 domain binding protein 1, SH3BGRL3, SH3BP 1, TIP B1
计算分子量 93 aa, 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC030135
基因名称 SH3BGRL3
Gene ID (NCBI) 83442
RRIDAB_10694666
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H299
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein, could act as a modulator of glutaredoxin biological activity. It may play a role in cytoskeleton organization (PMID: 34380438). SH3BGRL3 acts as a potential prognostic biomarker for urothelial carcinoma and a novel binding partner of epidermal growth factor receptor (PMID: 26286913).

实验方案

Product Specific Protocols
WB protocol for SH3BGRL3 antibody 12210-1-APDownload protocol
IHC protocol for SH3BGRL3 antibody 12210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Chem Biol

Citrullination/Methylation Crosstalk on Histone H3 Regulates ER-Target Gene Transcription.

Authors - Kathleen W Clancy
View Article