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验证数据展示

  • WB analysis using 12210-1-AP

    WB analysis using 12210-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 12210-1-AP

    IHC staining of human stomach cancer using 12210-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human stomach cancer using 12210-1-AP

    IHC staining of human stomach cancer using 12210-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12210-1-AP (SH3BGRL3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SH3BGRL3 Polyclonal antibody

SH3BGRL3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12210-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

SH3 domain binding protein 1, SH3BGRL3, SH3BP 1, TIP B1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inPC-3 cells, U-251 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12210-1-AP targets SH3BGRL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 SH3BGRL3 fusion protein Ag2857 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 domain binding glutamic acid-rich protein like 3
别名 SH3 domain binding protein 1, SH3BGRL3, SH3BP 1, TIP B1
计算分子量 93 aa, 10 kDa
观测分子量10 kDa
GenBank蛋白编号BC030135
基因名称 SH3BGRL3
Gene ID (NCBI) 83442
RRIDAB_10694666
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H299
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein, could act as a modulator of glutaredoxin biological activity. It may play a role in cytoskeleton organization (PMID: 34380438). SH3BGRL3 acts as a potential prognostic biomarker for urothelial carcinoma and a novel binding partner of epidermal growth factor receptor (PMID: 26286913).

实验方案

Product Specific Protocols
WB protocol for SH3BGRL3 antibody 12210-1-APDownload protocol
IHC protocol for SH3BGRL3 antibody 12210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

ACS Chem Biol

Citrullination/Methylation Crosstalk on Histone H3 Regulates ER-Target Gene Transcription.

Authors - Kathleen W Clancy
View Article