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  • Featured Product
  • KD/KO Validated

SH3BGRL2 Polyclonal antibody

SH3BGRL2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21944-1-AP

Print datasheet

Synonyms

FASH3, SH3BGRL2

验证数据展示

  • WB analysis of mouse brain using 21944-1-AP

    WB analysis of mouse brain using 21944-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21944-1-AP

    IHC staining of human liver using 21944-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 21944-1-AP

    IHC staining of human liver using 21944-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HEK-293 using 21944-1-AP

    IF Staining of HEK-293 using 21944-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, human placenta tissue
Positive IHC detected inhuman liver tissue, human placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21944-1-AP is SH3BGRL2 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen SH3BGRL2 fusion protein Ag16638 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SH3 domain binding glutamic acid-rich protein like 2
Synonyms FASH3, SH3BGRL2
Calculated Molecular Weight 107 aa, 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC040489
Gene Symbol SH3BGRL2
Gene ID (NCBI) 83699
RRIDAB_11182822
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UJC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3BGRL2 antibody 21944-1-APDownload protocol
IHC protocol for SH3BGRL2 antibody 21944-1-APDownload protocol
IF protocol for SH3BGRL2 antibody 21944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Cancer Res

SH3BGRL2 exerts a dual function in breast cancer growth and metastasis and is regulated by TGF-β1.

Authors - Dou-Dou Li
  • KD Validated
View Article
humanWB

Front Pharmacol

Astragalus Polysaccharides Inhibits Tumorigenesis and Lipid Metabolism Through miR-138-5p/SIRT1/SREBP1 Pathway in Prostate Cancer.

Authors - Shanqi Guo
View Article