• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of mouse brain using 21944-1-AP

    WB analysis of mouse brain using 21944-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 21944-1-AP

    IHC staining of human liver using 21944-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver using 21944-1-AP

    IHC staining of human liver using 21944-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of HEK-293 using 21944-1-AP

    IF Staining of HEK-293 using 21944-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 21944-1-AP (SH3BGRL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

SH3BGRL2 Polyclonal antibody

SH3BGRL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21944-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

SH3 domain-binding glutamic acid-rich-like protein 2, Fovea-associated SH3 domain-binding protein, FASH3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inmouse brain tissue, mouse liver tissue, human placenta tissue
Positive IHC detected inhuman liver tissue, human placenta tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21944-1-AP targets SH3BGRL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human
免疫原

CatNo: Ag16638

Product name: Recombinant human SH3BGRL2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-107 aa of BC040489

Sequence: MVIRVFIASSSGFVAIKKKQQDVVRFLEANKIEFEEVDTTMSEEQRQWMYKNVPPEKKPTQGNPLPPQIFNGDRYCGDYDSFFESKESNTVFSFLGLKPRLASKAEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 domain binding glutamic acid-rich protein like 2
别名 SH3 domain-binding glutamic acid-rich-like protein 2, Fovea-associated SH3 domain-binding protein, FASH3
计算分子量 107 aa, 12 kDa
观测分子量 12 kDa
GenBank蛋白编号BC040489
基因名称 SH3BGRL2
Gene ID (NCBI) 83699
RRIDAB_11182822
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SH3BGRL2 antibody 21944-1-APDownload protocol
IHC protocol for SH3BGRL2 antibody 21944-1-APDownload protocol
IF protocol for SH3BGRL2 antibody 21944-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Am J Cancer Res

SH3BGRL2 exerts a dual function in breast cancer growth and metastasis and is regulated by TGF-β1.

Authors - Dou-Dou Li
  • KD Validated
View Article
humanWB

Front Pharmacol

Astragalus Polysaccharides Inhibits Tumorigenesis and Lipid Metabolism Through miR-138-5p/SIRT1/SREBP1 Pathway in Prostate Cancer.

Authors - Shanqi Guo
View Article