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验证数据展示

  • WB analysis of mouse colon using 11253-1-AP (same clone as 11253-1-PBS)

    WB analysis of mouse colon using 11253-1-AP (same clone as 11253-1-PBS)

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 11253-1-AP (SH3BGRL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11253-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 11253-1-AP (same clone as 11253-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 11253-1-AP (same clone as 11253-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 11253-1-AP (SH3BGRL antibody) at dilution of 1:50 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11253-1-PBS in a different storage buffer formulation.

SH3BGRL Polyclonal antibody, PBS Only

SH3BGRL Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 11253-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11253-1-PBS targets SH3BGRL in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1780

Product name: Recombinant human SH3BGRL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-114 aa of BC016709

Sequence: MVIRVYIASSSGSTAIKKKQQDVLGFLEANKIGFEEKDIAANEENRKWMRENVPENSRPATGYPLPPQIFNESQYRGDYDAFFEARENNAVYAFLGLTAPPGSKEAEVQAKQQA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SH3 domain binding glutamic acid-rich protein like
别名
计算分子量 13 kDa
观测分子量13 kDa
GenBank蛋白编号BC016709
基因名称 SH3BGRL
Gene ID (NCBI) 6451
RRIDAB_3085369
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75368
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SH3BGRL, an adaptor protein, is one member of the SH3BGR family. SH3BGRL is ubiquitously expressed in varied human tissues and organs, including the bone marrow, heart, lung, liver, and kidney. SH3BGRL encodes a small protein with a conserved proline-rich PLPPQIF region and two motifs, Homer EVH1-binding and SH3-binding motifs. As a scaffold protein, SH3BGRL could participate in the protein-protein interaction for the integration or/and crosstalk of signal transduction, membrane trafficking, cytoskeletal rearrangements, and other key cellular processes.

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