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SGSM3 Polyclonal antibody

SGSM3 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27147-1-AP

Print datasheet

Synonyms

MAP, Merlin associated protein, RabGAPLP, RUSC3, RUTBC3, SGSM3

验证数据展示

  • WB analysis using 27147-1-AP

    WB analysis using 27147-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27147-1-AP (SGSM3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 27147-1-AP

    IHC staining of human breast cancer using 27147-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27147-1-AP (SGSM3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 27147-1-AP

    IHC staining of human breast cancer using 27147-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27147-1-AP (SGSM3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inA549 cells, mouse brain tissue, HT-1376 cells, L02 cells, rat brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27147-1-AP targets SGSM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SGSM3 fusion protein Ag25954 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name small G protein signaling modulator 3
Synonyms MAP, Merlin associated protein, RabGAPLP, RUSC3, RUTBC3, SGSM3
Calculated Molecular Weight 749 aa, 85 kDa
Observed Molecular Weight 85 kDa
GenBank Accession NumberBC008078
Gene Symbol SGSM3
Gene ID (NCBI) 27352
RRIDAB_2880774
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96HU1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SGSM3 antibody 27147-1-APDownload protocol
IHC protocol for SGSM3 antibody 27147-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols