验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, PC-3 cells, NIH/3T3 cells, mouse heart tissue, rat heart tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16977-1-AP targets SGOL1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB, IF, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | SGOL1 fusion protein Ag9700 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | shugoshin-like 1 (S. pombe) |
别名 | hSgo1, NY BR 85, SGO, Sgo1, SGOL1, Shugoshin like 1, shugoshin like 1 (S. pombe) |
计算分子量 | 561aa,64 kDa; 292aa,34 kDa |
观测分子量 | 64 kDa |
GenBank蛋白编号 | BC017867 |
基因名称 | SGOL1 |
Gene ID (NCBI) | 151648 |
RRID | AB_2188437 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q5FBB7 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SGOL1 (Shugoshin 1), a centromeric protein, plays an important role in mitosis (PMID: 23102702).
实验方案
Product Specific Protocols | |
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WB protocol for SGOL1 antibody 16977-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Carcinogenesis Tumor-promoting/progressing role of additional chromosome instability in hepatic carcinogenesis in Sgo1 (Shugoshin 1) haploinsufficient mice. | ||
Front Oncol Deciphering the Role of Shugoshin-Like Protein 1 in Lung Adenocarcinoma: A Comprehensive Analysis and In Vitro Study. | ||
Mol Carcinog Antagonizing pathways leading to differential dynamics in colon carcinogenesis in Shugoshin1 (Sgo1)-haploinsufficient chromosome instability model. | ||
BMC Med Genomics Prognosis and biological function of SGOL1 in clear cell renal cell carcinoma: a multiomics analysis
| ||
Front Oncol Identification and validation of a potential key gene SGOL1 for poor prognosis in hepatocellular carcinoma based on a bioinformatics approach | ||
J Mol Cell Biol Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis
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