验证数据展示
经过测试的应用
Positive WB detected in | HT-29 cells, human heart tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
19050-1-AP targets SGMS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | SGMS1 fusion protein Ag5246 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | sphingomyelin synthase 1 |
别名 | hmob33, MOB, MOB1, Protein Mob, SGMS1, SMS1, sphingomyelin synthase 1, TMEM23, Transmembrane protein 23 |
计算分子量 | 49 kDa |
观测分子量 | 25 kDa, 49 kDa |
GenBank蛋白编号 | BC042899 |
基因名称 | SGMS1 |
Gene ID (NCBI) | 259230 |
RRID | AB_2188417 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q86VZ5 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sphingomyelin synthase (SMS), with two isoforms, SMS1 (SGMS1) and SMS2, is a key enzyme involved in the generation and development of SM. SMS can participate in inflammation, atherosclerosis, proliferation, apoptosis, differentiation and other functions(PMID:30535436). SMS1 is frequently downregulated in various solid cancers, more particularly in melanoma(PMID:31114500). SGMS1 has two isoforms.
实验方案
Product Specific Protocols | |
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WB protocol for SGMS1 antibody 19050-1-AP | Download protocol |
IHC protocol for SGMS1 antibody 19050-1-AP | Download protocol |
IP protocol for SGMS1 antibody 19050-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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FASEB J Sphingomyelin maintains the cutaneous barrier via regulation of the STAT3 pathway. | ||
Exp Neurol Inhibition of sphingomyelin synthase 1 ameliorates alzheimer-like pathology in APP/PS1 transgenic mice through promoting lysosomal degradation of BACE1.
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iScience The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways. | ||
Drug Des Devel Ther Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease. |