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SGMS1 Polyclonal antibody

SGMS1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 19050-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

hmob33, MOB, MOB1, Protein Mob, SGMS1, SMS1, sphingomyelin synthase 1, TMEM23, Transmembrane protein 23

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHT-29 cells, human heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19050-1-AP targets SGMS1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 SGMS1 fusion protein Ag5246 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sphingomyelin synthase 1
别名 hmob33, MOB, MOB1, Protein Mob, SGMS1, SMS1, sphingomyelin synthase 1, TMEM23, Transmembrane protein 23
计算分子量 49 kDa
观测分子量 25 kDa, 49 kDa
GenBank蛋白编号BC042899
基因名称 SGMS1
Gene ID (NCBI) 259230
RRIDAB_2188417
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86VZ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sphingomyelin synthase (SMS), with two isoforms, SMS1 (SGMS1) and SMS2, is a key enzyme involved in the generation and development of SM. SMS can participate in inflammation, atherosclerosis, proliferation, apoptosis, differentiation and other functions(PMID:30535436). SMS1 is frequently downregulated in various solid cancers, more particularly in melanoma(PMID:31114500). SGMS1 has two isoforms.

实验方案

Product Specific Protocols
WB protocol for SGMS1 antibody 19050-1-APDownload protocol
IHC protocol for SGMS1 antibody 19050-1-APDownload protocol
IP protocol for SGMS1 antibody 19050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Cancer Res

Aneuploid cell survival relies upon sphingolipid homeostasis.

Authors - Yun-Chi Tang
  • KD Validated
humanIHC

J Clin Endocrinol Metab

Dysregulated sphingolipid metabolism in endometriosis.

Authors - Yie Hou Lee
mouseWB,IHC

FASEB J

Sphingomyelin maintains the cutaneous barrier via regulation of the STAT3 pathway.

Authors - Mariko Komuro
human,mouseWB,IF

Exp Neurol

Inhibition of sphingomyelin synthase 1 ameliorates alzheimer-like pathology in APP/PS1 transgenic mice through promoting lysosomal degradation of BACE1.

Authors - Mei-Hong Lu
  • KD Validated
mouseWB

iScience

The Symbiotic Relationship between the Neural Retina and Retinal Pigment Epithelium Is Supported by Utilizing Differential Metabolic Pathways.

Authors - Tirthankar Sinha
mouseIF

Drug Des Devel Ther

Involvement of Huanglian Jiedu Decoction on Microglia with Abnormal Sphingolipid Metabolism in Alzheimer's Disease.

Authors - Yi-Yu Qi
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