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验证数据展示

  • WB analysis of mouse kidney using 24327-1-AP (same clone as 24327-1-PBS)

    WB analysis of mouse kidney using 24327-1-AP (same clone as 24327-1-PBS)

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24327-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 24327-1-AP (same clone as 24327-1-PBS)

    IHC staining of human kidney using 24327-1-AP (same clone as 24327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:50. This data was developed using the same antibody clone with 24327-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 24327-1-AP (same clone as 24327-1-PBS)

    IHC staining of human kidney using 24327-1-AP (same clone as 24327-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 24327-1-AP (SGLT3/SLC5A4 antibody at dilution of 1:50. This data was developed using the same antibody clone with 24327-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse kidney using 24327-1-AP (same clone as 24327-1-PBS)

    IP experiment of mouse kidney using 24327-1-AP (same clone as 24327-1-PBS)

    IP result of anti-SGLT3/SLC5A4 (IP:24327-1-AP, 4ug; Detection:24327-1-AP 1:500) with mouse kidney tissue lysate 4000ug. This data was developed using the same antibody clone with 24327-1-PBS in a different storage buffer formulation.

SGLT3/SLC5A4 Polyclonal antibody, PBS Only

SGLT3/SLC5A4 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 24327-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24327-1-PBS targets SGLT3/SLC5A4 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19391

Product name: Recombinant human SLC5A4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 579-633 aa of BC153069

Sequence: SQEETDDGVEEDYPEKSRGCLKKAYDLFCGLQKGPKLTKEEEEALSKKLTDTSER

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 5 (low affinity glucose cotransporter), member 4
别名
计算分子量 659 aa, 72 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC153069
基因名称 SGLT3
Gene ID (NCBI) 6527
RRIDAB_2650519
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY91
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC5A4, also known as SGLT3, is a sugar sensor that depolarizes the plasma membrane in response to external glucose (PMID: 12748858, 20421923). SGLT3 mediates the influx of sodium ions into the cell but does not transport sugars, also potently activated by imino sugars such as deoxynojirimycin (PMID: 22766068, 13130073). SGLT3 is a plasma membrane protein, shares 70% identity with SGLT1, and is expressed in the small intestine (cholinergic neurons), skeletal muscle, kidney, uterus, and testis (PMID: 12748858). SGLT3 protein at apparent molecular masses of 72 kDa and 80 kDa in human kidney tissue and HK-2 cells (PMID: 22766068).

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