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验证数据展示

  • WB analysis of human placenta using 11185-1-AP

    WB analysis of human placenta using 11185-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 11185-1-AP (SGK2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11185-1-AP

    IHC staining of human pancreas cancer using 11185-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11185-1-AP (SGK2 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas cancer using 11185-1-AP

    IHC staining of human pancreas cancer using 11185-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11185-1-AP (SGK2 Antibody) at dilution of 1:200 (under 40x lens).

  • Featured Product
  • KD/KO验证

SGK2 Polyclonal antibody

SGK2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 11185-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EC:2.7.11.1, H SGK2, Serine/threonine-protein kinase Sgk2, Serum/glucocorticoid-regulated kinase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue, mouse pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11185-1-AP targets SGK2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原

CatNo: Ag1669

Product name: Recombinant human SGK2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-396 aa of BC014037

Sequence: EEGRAVHGAAPQDLSRNLEFSELPDHCYRMNSSPAGTPSPQPSRANGNINLGPSANPNAQPTDFDFLKVIGKGNYGKVLLAKRKSDGAFYAVKVLQKKSILKKKEQSHIMAERSVLLKNVRHPFLVGLRYSFQTPEKLYFVLDYVNGGELFFHLQRERRFLEPRARFYAAEVASAIGYLHSLNIIYRDLKPENILLDCQGHVVLTDFGLCKEGVEPEDTTSTFCGTPEYLAPEVLRKEPYDRAVDWWCLGAVLYEMLHGLPPFYSQDVSQMYENILHQPLQIPGGRTVAACDLLQSLLHKDQRQRLGSKADFLEIKNHVFFSPINWDDLYHKRLTPPFNPNVTGPADLKHFDPEFTQEAVSKSIGCTPDTVASSSGASSAFLGFSYAPEDDDILDC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serum/glucocorticoid regulated kinase 2
别名 EC:2.7.11.1, H SGK2, Serine/threonine-protein kinase Sgk2, Serum/glucocorticoid-regulated kinase 2
计算分子量 48 kDa
观测分子量41-48 kDa
GenBank蛋白编号BC014037
基因名称 SGK2
Gene ID (NCBI) 10110
RRIDAB_2188525
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBY8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SGK2 antibody 11185-1-APDownload protocol
IHC protocol for SGK2 antibody 11185-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Autophagy

Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits.

Authors - Seonghee Jung
  • KO Validated
View Article