SGK1 Polyclonal antibody
SGK1 Polyclonal Antibody for FC, IF/ICC, IHC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
FC, IF/ICC, IHC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive IP detected in | HEK-293 cells |
Positive IHC detected in | human pancreas tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HEK-293 cells |
Positive FC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 1 publications below |
产品信息
The immunogen of 23394-1-AP is SGK1 Fusion Protein expressed in E. coli.
Tested Applications | FC, IF/ICC, IHC, IP, ELISA |
Cited Applications | IHC |
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Immunogen | SGK1 fusion protein Ag17888 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | serum/glucocorticoid regulated kinase 1 |
Synonyms | SGK, SGK1 |
Calculated Molecular Weight | 431 aa, 49 kDa |
GenBank Accession Number | BC001263 |
Gene Symbol | SGK1 |
Gene ID (NCBI) | 6446 |
RRID | AB_2879271 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O00141 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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IHC protocol for SGK1 antibody 23394-1-AP | Download protocol |
IF protocol for SGK1 antibody 23394-1-AP | Download protocol |
IP protocol for SGK1 antibody 23394-1-AP | Download protocol |
FC protocol for SGK1 antibody 23394-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
FASEB J Inhibition of serum- and glucocorticoid-inducible kinase 1 enhances TLR-mediated inflammation and promotes endotoxin-driven organ failure. | ||
J Innate Immun Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery. | ||
J Immunol Serum- and Glucocorticoid-Inducible Kinase 1 Promotes Alternative Macrophage Polarization and Restrains Inflammation through FoxO1 and STAT3 Signaling. | ||
J Transl Med Hepatocyte SGK1 activated by hepatic ischemia-reperfusion promotes the recurrence of liver metastasis via IL-6/STAT3 | ||
Appl Biochem Biotechnol Long Non-coding RNA ZFAS1 Regulates Fibrosis and Scortosis in the Cell Model of Diabetic Nephropathy Through miR-525-5p/SGK1 Axis |