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SGCE Polyclonal antibody

SGCE Polyclonal Antibody for IF/ICC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 12407-1-AP

Print datasheet

Synonyms

DYT11, Epsilon sarcoglycan, Epsilon SG, ESG, sarcoglycan, epsilon, SGCE, ε-sarcoglycan

验证数据展示

  • WB analysis of mouse brain using 12407-1-AP

    WB analysis of mouse brain using 12407-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12407-1-AP (SGCE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse heart using 12407-1-AP

    IP experiment of mouse heart using 12407-1-AP

    IP result of anti-SGCE (IP:12407-1-AP, 4ug; Detection:12407-1-AP 1:500) with mouse heart tissue lysate 4000ug.

  • IF Staining of HeLa using 12407-1-AP

    IF Staining of HeLa using 12407-1-AP

    Immunofluorescent analysis of HeLa cells using 12407-1-AP (SGCE antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, human brain tissue, human heart tissue, mouse heart tissue
Positive IP detected inmouse heart tissue
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

12407-1-AP targets SGCE in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen SGCE fusion protein Ag3118 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sarcoglycan, epsilon
Synonyms DYT11, Epsilon sarcoglycan, Epsilon SG, ESG, sarcoglycan, epsilon, SGCE, ε-sarcoglycan
Calculated Molecular Weight 437 aa, 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC021709
Gene Symbol SGCE
Gene ID (NCBI) 8910
RRIDAB_2190443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43556
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SGCE antibody 12407-1-APDownload protocol
IF protocol for SGCE antibody 12407-1-APDownload protocol
IP protocol for SGCE antibody 12407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Biochem

Earlier onset of motor deficits in mice with double mutations in Dyt1 and Sgce.

Authors - Fumiaki Yokoi
View Article