验证数据展示
经过测试的应用
| Positive WB detected in | human skeletal muscle tissue |
Planning an IHC experiment? We recommend our IHCeasy SGCA Ready-To-Use IHC Kit. SGCA primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13222-1-AP targets Alpha sarcoglycan in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Alpha sarcoglycan fusion protein Ag3924 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein) |
| 别名 | 50 DAG, 50DAG, A2, adhalin, ADL, Alpha sarcoglycan, Alpha SG, DAG2, DMDA2, Dystroglycan 2, LGMD2D, SCARMD1, SGCA |
| 计算分子量 | 387 aa, 43 kDa |
| GenBank蛋白编号 | BC025702 |
| 基因名称 | alpha Sarcoglycan |
| Gene ID (NCBI) | 6442 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q16586 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Alpha sarcoglycan antibody 13222-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |