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验证数据展示

  • WB analysis of human skeletal muscle using 13222-1-AP

    WB analysis of human skeletal muscle using 13222-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13222-1-AP (Alpha-sarcoglycan antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

Alpha Sarcoglycan Polyclonal antibody

Alpha sarcoglycan Polyclonal Antibody for WB, ELISA
Cat No. 13222-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

50 DAG, 50DAG, A2, adhalin, ADL, Alpha sarcoglycan, Alpha SG, DAG2, DMDA2, Dystroglycan 2, LGMD2D, SCARMD1, SGCA

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman skeletal muscle tissue
Planning an IHC experiment? We recommend our IHCeasy SGCA Ready-To-Use IHC Kit. SGCA primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13222-1-AP targets Alpha sarcoglycan in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3924

Product name: Recombinant human SGCA protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 25-289 aa of BC025702

Sequence: QTTLHPLVGRVFVHTLDHETFLSLPEHVAVPPAVHITYHAHLQGHPDLPRWLRYTQRSPHHPGFLYGSATPEDRGLQVIEVTAYNRDSFDTTRQRLVLEIGDPEGPLLPYQAEFLVRSHDAEEVLPSTPASRFLSALGGLWEPGELQLLNVTSALDRGGRVPLPIEGRKEGVYIKVGSASPFSTCLKMVASPDSHARCAQGQPPLLSCYDTLAPHFRVDWCNVTLVDKSVPEPADEVPTPGDGILEHDPFFCPPTEAPDRDFLVD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sarcoglycan, alpha (50kDa dystrophin-associated glycoprotein)
别名 50 DAG, 50DAG, A2, adhalin, ADL, Alpha sarcoglycan, Alpha SG, DAG2, DMDA2, Dystroglycan 2, LGMD2D, SCARMD1, SGCA
计算分子量 387 aa, 43 kDa
GenBank蛋白编号BC025702
基因名称 alpha Sarcoglycan
Gene ID (NCBI) 6442
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16586
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-sarcoglycan is a member of the sarcoglycan-sarcospan complex (SG-SSPN), which is constituted by α/ɛ-, β-, γ-, and δ-SGs as well as SSPN. Alpha-sarcoglycan gene expression is exclusive of striated muscle and is finely regulated during myogenic differentiation, where the amount of alpha-sarcoglycan transcript is selectively increased after differentiation to myotubes (MTs). Alpha sarcoglycan's disruption causes limb-girdle muscular dystrophy.

实验方案

Product Specific Protocols
WB protocol for Alpha sarcoglycan antibody 13222-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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