验证数据展示
经过测试的应用
Positive WB detected in | PC-3 cells, LNCaP cells, MCF-7 cells, HepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, Rat brain tissue, Mouse brain tissue |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68024-1-Ig targets SFXN1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
经测试应用 | WB, IF/ICC, ELISA Application Description |
经测试反应性 | Human, Rat, Mouse |
免疫原 | SFXN1 fusion protein Ag2985 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | sideroflexin 1 |
别名 | FLJ12876, SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein |
计算分子量 | 35.6 kDa |
观测分子量 | 32 kDa |
GenBank蛋白编号 | BC020517 |
基因名称 | SFXN1 |
Gene ID (NCBI) | 94081 |
RRID | AB_2918768 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | Q9H9B4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.
实验方案
Product Specific Protocols | |
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WB protocol for SFXN1 antibody 68024-1-Ig | Download protocol |
IF protocol for SFXN1 antibody 68024-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |