验证数据展示
经过测试的应用
Positive WB detected in | MCF-7 cells, human brain tissue, mouse liver tissue, mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
12296-1-AP targets SFXN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat |
免疫原 | SFXN1 fusion protein Ag2985 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | sideroflexin 1 |
别名 | SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein |
计算分子量 | 35.6 kDa |
观测分子量 | 30-35 kDa |
GenBank蛋白编号 | BC020517 |
基因名称 | SFXN1 |
Gene ID (NCBI) | 94081 |
RRID | AB_2185814 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9H9B4 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.
实验方案
Product Specific Protocols | |
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WB protocol for SFXN1 antibody 12296-1-AP | Download protocol |
IHC protocol for SFXN1 antibody 12296-1-AP | Download protocol |
IP protocol for SFXN1 antibody 12296-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Chem Biol Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.
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J Proteome Res The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus. | ||
Cell Rep The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.
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Front Immunol YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis | ||
Sci Rep Loss of SFXN1 mitigates lipotoxicity and predicts poor outcome in non-viral hepatocellular carcinoma | ||
Sci Total Environ Ferritinophagy activation and sideroflexin1-dependent mitochondrial iron overload contribute to patulin-induced cardiac inflammation and fibrosis
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