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SFXN1 Polyclonal antibody

SFXN1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 12296-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inMCF-7 cells, human brain tissue, mouse liver tissue, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12296-1-AP targets SFXN1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 SFXN1 fusion protein Ag2985 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sideroflexin 1
别名 SFXN1, sideroflexin 1, TCC, Tricarboxylate carrier protein
计算分子量35.6 kDa
观测分子量 30-35 kDa
GenBank蛋白编号BC020517
基因名称 SFXN1
Gene ID (NCBI) 94081
RRIDAB_2185814
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H9B4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SFXN1, the founding member of the SLC56 family, was identified by positional cloning of the mutation in the flexed-tail mouse model with sideroblastic anemia. SFXN1 was recently identified as a mitochondrial serine transporter essential for one-carbon (1C) metabolism, a process in which folate species are generated and used in biosynthetic pathways required for cell proliferation. In HEK293 cells, SFXN1 is the most abundant isoform among the SFXNs.

实验方案

Product Specific Protocols
WB protocol for SFXN1 antibody 12296-1-APDownload protocol
IHC protocol for SFXN1 antibody 12296-1-APDownload protocol
IP protocol for SFXN1 antibody 12296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Metabolic determinants of cancer cell sensitivity to canonical ferroptosis inducers.

Authors - Mariluz Soula
  • KO Validated
mouseIHC,IF,IP

J Proteome Res

The liver connexin32 interactome is a novel plasma membrane-mitochondrial signaling nexus.

Authors - Fowler Stephanie L SL
humanWB

Cell Rep

The mitochondrial carrier SFXN1 is critical for complex III integrity and cellular metabolism.

Authors - Michelle Grace Acoba
  • KO Validated
mouseWB

Front Immunol

YAP1 alleviates sepsis-induced acute lung injury via inhibiting ferritinophagy-mediated ferroptosis

Authors - Jing Zhang
humanIHC,WB

Sci Rep

Loss of SFXN1 mitigates lipotoxicity and predicts poor outcome in non-viral hepatocellular carcinoma

Authors - Kohei Yagi
mouse,ratIHC,WB

Sci Total Environ

Ferritinophagy activation and sideroflexin1-dependent mitochondrial iron overload contribute to patulin-induced cardiac inflammation and fibrosis

Authors - Wen-Chang Sun
  • KD Validated
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