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- KD/KO Validated
SFRS7 Polyclonal antibody
SFRS7 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, mouse testis tissue, rat testis tissue, HeLa cells, HepG2 cells, SKOV-3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SRSF7/SFRS7 Ready-To-Use IHC Kit. SRSF7/SFRS7 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11044-1-AP targets SFRS7 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | SFRS7 fusion protein Ag1526 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | splicing factor, arginine/serine-rich 7, 35kDa |
| Synonyms | 9G8, AAG3, SFRS7, Splicing factor 9G8, SRSF7 |
| Calculated Molecular Weight | 27 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC000997 |
| Gene Symbol | SFRS7 |
| Gene ID (NCBI) | 6432 |
| RRID | AB_2185229 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16629 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SFRS7 is a member of the serine/arginine (SR) splicing factor family, which mediated the alternative splicing events target the resulting mRNAs for degradation by means of an RNA surveillance pathway called nonsense-mediated mRNA decay. It was found to repress the splicing of MAPT/Tau exon 10
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SFRS7 antibody 11044-1-AP | Download protocol |
| IHC protocol for SFRS7 antibody 11044-1-AP | Download protocol |
| IF protocol for SFRS7 antibody 11044-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Cell C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles. | ||
Nucleic Acids Res Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.
| ||
Nucleic Acids Res Exonic splicing code and protein binding sites for calcium.
| ||
Genomics Proteomics Bioinformatics Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.
| ||
Genes Genomics SRSF7 is a promising prognostic biomarker in hepatocellular carcinoma and is associated with immune infiltration
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