验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
产品信息
11772-1-AP targets SFRS6 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | SFRS6 fusion protein Ag2412 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | splicing factor, arginine/serine-rich 6 |
别名 | B52, Pre mRNA splicing factor SRP55, SFRS6, SRP55, SRSF6 |
计算分子量 | 344 aa, 40 kDa |
观测分子量 | 40 kDa |
GenBank蛋白编号 | BC006832 |
基因名称 | SFRS6 |
Gene ID (NCBI) | 6431 |
RRID | AB_2185212 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13247 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SR proteins have a characteristic C-terminal Ser/Arg-rich repeat (RS domain) of variable length and constitute a family of highly conserved nuclear phosphoproteins that can function as both essential and alternative pre-mRNA splicing factors [PMID:7556075]. SRFS6 has a pivotal role in the TF exon 5 definition, an event essential for flTF biosynthesis. Besides, up- or down regulation of SRSF6 alters the splicing of several tumor suppressors and oncogenes to generate the oncogenic isoforms and reduce the tumor suppressive isoforms [PMID:23132731].
实验方案
Product Specific Protocols | |
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WB protocol for SFRS6 antibody 11772-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth. | ||
J Cell Mol Med Renal Tubular Epithelial Cell-Derived hsa_circ_0008925 From Urine Is Related to Chronic Renal Fibrosis | ||
Gene Intron retention as a new pre-symptomatic marker of aging and its recovery to the normal state by a traditional Japanese multi-herbal medicine. | ||
Data Brief Data of RNA-seq transcriptomes of liver, bone, heart, kidney and blood in klotho mice at a pre-symptomatic state and the effect of a traditional Japanese multi-herbal medicine, juzentaihoto. |