验证数据展示
经过测试的应用
| Positive IHC detected in | human cervical cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
This antibody is recommended for IHC.
Planning an IHC experiment? We recommend our IHCeasy SRSF3 Ready-To-Use IHC Kit. SRSF3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IHC | See 2 publications below |
产品信息
10916-1-AP targets SRp20 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, rat |
| 免疫原 | SRp20 fusion protein Ag1341 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | splicing factor, arginine/serine-rich 3 |
| 别名 | Pre mRNA splicing factor SRP20, SFRS3, SRp20, SRSF3 |
| 计算分子量 | 20 kDa |
| GenBank蛋白编号 | BC000914 |
| 基因名称 | SRSF3 |
| Gene ID (NCBI) | 6428 |
| RRID | AB_2185064 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P84103 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SFRS3, also named as SRSF3 and SRP20, belongs to the splicing factor SR family. It may be involved in RNA processing in relation with cellular proliferation and/or maturation. SFRS3 is one of the cognate factors. It is a proto-oncogene critical for cell proliferation and tumor induction and maintenance.The MW of SFRS3 is 20kd.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for SRp20 antibody 10916-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Mol Cell Cross-Regulation between TDP-43 and Paraspeckles Promotes Pluripotency-Differentiation Transition. | ||
Front Oncol SRSF3 Expression Serves as a Potential Biomarker for Prognostic and Immune Response in Pan-Cancer. | ||
Pathol Res Pract LINC01210 promotes malignant phenotypes of colorectal cancer through epigenetically upregulating SRSF3. | ||
Rev Port Cardiol Mesenchymal stem cells may alleviate angiotensin II-induced myocardial fibrosis and hypertrophy by upregulating SFRS3 expression |